N-aminomorpholine-functionalized bromine-doped carbon dots for hypochlorous acid detection in foods and imaging in live cells

Chengda Zhao; Pengxiang Yuan; Dan Wang; Shiyao Li; Huan Yao; Liu-Pan Yang; Li-Li Wang; Fangfang Du

doi:10.1016/j.foodchem.2023.138284

N-aminomorpholine-functionalized bromine-doped carbon dots for hypochlorous acid detection in foods and imaging in live cells

Food Chem. 2024 May 30:441:138284. doi: 10.1016/j.foodchem.2023.138284. Epub 2023 Dec 28.

Authors

Chengda Zhao¹, Pengxiang Yuan², Dan Wang², Shiyao Li², Huan Yao², Liu-Pan Yang², Li-Li Wang³, Fangfang Du⁴

Affiliations

¹ The Affiliated Nanhua Hospital, Department of Anesthesiology, Hengyang Medical School, University of South China, Hengyang, Hunan 421001, China.
² School of Pharmaceutical Science, Hengyang Medical School, University of South China, Hengyang, Hunan 421001, China.
³ School of Pharmaceutical Science, Hengyang Medical School, University of South China, Hengyang, Hunan 421001, China. Electronic address: wangll@usc.edu.cn.
⁴ Key Laboratory of Hainan Trauma and Disaster Rescue, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou 571199, China; Engineering Research Center for Hainan Bio-Smart Materials and Bio-Medical Devices, Key Laboratory of Emergency and Trauma, Ministry of Education, Key Laboratory of Hainan Functional Materials and Molecular Imaging, College of Emergency and Trauma, Hainan Medical University, Haikou 571199, China. Electronic address: dfang@usc.edu.cn.

PMID: 38181668
DOI: 10.1016/j.foodchem.2023.138284

Abstract

Hypochlorous acid (HClO) is used in food preservation. However, excessive HClO can deteriorate nutritional composition of food, compromise its quality, and potentially induce various diseases. Consequently, the development of multifunctional fluorescent probes for the sensitive and selective detection of HClO is highly anticipated for food safety. In this work, we designed a nanoprobe using N-aminomorpholine (AM)-functionalized bromine-doped carbon dots (Br-CDs-AM) for sensing HClO. This nanoprobe exhibits pH stability, strong resistance to photobleaching, superior long-term photostability (12 weeks), high sensitivity (19.3 nM), and an ultrarapid response (8 s) for detecting HClO residues in food matrices with percentage recovery (96.5 %-108 %) and RSDs less than 5.34 %. In addition, extremely low cytotoxicity and outstanding biocompatibility enable the nanoprobe to be used primarily for lysosome tracking and rapidly visualizing HClO in live cells. Thus, this study provides a new pathway to design unconventional nanoprobes for food safety assessment and subcellular organelle-specific imaging HClO.

Keywords: Bromine-doped carbon dots; Fluorescent probe; Food safety; HClO sensing; Lysosome-targetable imaging; N-aminomorpholine-functionalized.

MeSH terms

Bromine*
Carbon / metabolism
Fluorescent Dyes / chemistry
Humans
Hypochlorous Acid* / chemistry
Hypochlorous Acid* / metabolism
Lysosomes / metabolism

Substances

Hypochlorous Acid
Bromine
Carbon
Fluorescent Dyes