DNA structural changes (photo)induced by tricarbonyl (pterin)rhenium(I) complex

F Ragone; J G Yañuk; F M Cabrerizo; E Prieto; E Wolcan; G T Ruiz

doi:10.1016/j.jinorgbio.2023.112471

DNA structural changes (photo)induced by tricarbonyl (pterin)rhenium(I) complex

J Inorg Biochem. 2024 Mar:252:112471. doi: 10.1016/j.jinorgbio.2023.112471. Epub 2023 Dec 24.

Authors

F Ragone¹, J G Yañuk², F M Cabrerizo³, E Prieto⁴, E Wolcan¹, G T Ruiz⁵

Affiliations

¹ Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA, UNLP, CCT La Plata-CONICET), Diag. 113 y 64, Sucursal 4, C.C. 16, (B1906ZAA) La Plata, Argentina.
² Instituto Tecnológico de Chascomús (CONICET-UNSAM), Av. Intendente Marino Km 8.2, CC 164 (B7130IWA), Chascomús, Argentina; Escuela de Bio y Nanotecnologías (UNSAM), Argentina.
³ Instituto Tecnológico de Chascomús (CONICET-UNSAM), Av. Intendente Marino Km 8.2, CC 164 (B7130IWA), Chascomús, Argentina; Escuela de Bio y Nanotecnologías (UNSAM), Argentina. Electronic address: fcabrerizo@intech.gov.ar.
⁴ Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA, UNLP, CCT La Plata-CONICET), Diag. 113 y 64, Sucursal 4, C.C. 16, (B1906ZAA) La Plata, Argentina; ICS-UNAJ, Avenida Calchaqui 6200 Florencio Varela, Argentina.
⁵ Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA, UNLP, CCT La Plata-CONICET), Diag. 113 y 64, Sucursal 4, C.C. 16, (B1906ZAA) La Plata, Argentina. Electronic address: gruiz@inifta.unlp.edu.ar.

PMID: 38181612
DOI: 10.1016/j.jinorgbio.2023.112471

Abstract

We report on interactions of different types of DNA molecules including double-stranded and plasmid DNA as well as polynucleotides (poly[dGdC]₂ and poly[dAdT]₂) with fac-[Re^I(CO)₃(pterin)(H₂O)] (or Reptr) complex. The interaction was characterized spectroscopically and changes in the plasmid structure were verified by both electrophoresis and AFM microscopy. For comparative reasons, two others related tricarbonyl rhenium(I) complexes, fac-[(4,4'-bpy)Re^I(CO)₃(dppz)]⁺ (or Redppz) and fac-[(CF₃SO₃)Re^I(CO)₃(2,2'-bpy)] (or Rebpy) were also studied to further explore the influence of the different co-ligands on the interaction and DNA (photo)damage. Data reported herein suggests that DNA molecules can be structurally modified either by direct interaction with Re(I) complexes in their ground states inducing DNA relaxation, and/or through photoinduced cross-linking processes. The chemical nature of the co-ligands modulates the extent of the damage observed.

Keywords: AFM; DNA; Metallointercalator; Photoinduced processes; Rhenium - Pterin.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA / chemistry
Ligands
Plasmids
Pterins*
Rhenium* / chemistry

Substances

Pterins
Rhenium
DNA
Ligands