Neodothiora pruni sp. nov., a Biosurfactant-Producing Ascomycetous Yeast Species Isolated from Flower of Prunus mume

Jeong-Seon Kim; Miran Lee; Jun Heo; Soon-Wo Kwon; Bong-Sik Yun; Yiseul Kim

doi:10.1080/12298093.2023.2282257

Neodothiora pruni sp. nov., a Biosurfactant-Producing Ascomycetous Yeast Species Isolated from Flower of Prunus mume

Mycobiology. 2023 Dec 27;51(6):388-392. doi: 10.1080/12298093.2023.2282257. eCollection 2023.

Authors

Jeong-Seon Kim¹, Miran Lee¹, Jun Heo¹, Soon-Wo Kwon¹, Bong-Sik Yun², Yiseul Kim¹

Affiliations

¹ Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju, Republic of Korea.
² Division of Biotechnology and Advanced institute of Environmental and Bioscience, College of Environmental and Bioresource Sciences, Jeonbuk National University, Iksan, Republic of Korea.

Abstract

A yeast strain, designated as JAF-11^T, was isolated from flower of Prunus mume Sieb. et Zucc. in Gwangyang, Republic of Korea. Phylogenetic analysis showed that strain JAF-11^T was closely related to Neodothiora populina CPC 39399^T with 2.07 % sequence divergence (12 nucleotide substitutions and three gaps in 581 nucleotides) in the D1/D2 domain of the large subunit (LSU) rRNA gene, and Rhizosphaera macrospora CBS 208.79^T with 4.66 % sequence divergence (25 nucleotide substitutions and five gaps in 535 nucleotides) in the internal transcribed spacer (ITS) region. Further analysis based on the concatenated sequences of the D1/D2 domain of the LSU rRNA gene and the ITS region confirmed that strain JAF-11^T was well-separated from Neodothiora populina CPC 39399^T. In addition to the phylogenetic differences, strain JAF-11^T was distinguished from its closest species, Neodothiora populina CPC 39399^T and Rhizosphaera macrospora CBS 208.79^T belonging to the family Dothioraceae by its phenotypic characteristics, such as assimilation of carbon sources. Hence, the name Neodothiora pruni sp. nov. is proposed with type strain JAF-11^T (KACC 48808^T; MB 850034).

Keywords: Ascomycetous yeast; biosurfactant; novel yeast species.

Grants and funding

This study was carried out with the support (PJ015675) of the National Institute of Agricultural Sciences, Rural Development Administration, Republic of Korea.