Continuous Expression of Interferon Regulatory Factor 4 Sustains CD8+ T Cell Immunity against Tumor

Anze Yu; Jinfei Fu; Zheng Yin; Hui Yan; Xiang Xiao; Dawei Zou; Xiaolong Zhang; Xiongbing Zu; Xian C Li; Wenhao Chen

doi:10.34133/research.0271

Continuous Expression of Interferon Regulatory Factor 4 Sustains CD8⁺ T Cell Immunity against Tumor

Research (Wash D C). 2023 Nov 17:6:0271. doi: 10.34133/research.0271. eCollection 2023.

Authors

Anze Yu^{1

2}, Jinfei Fu¹, Zheng Yin^{3

4}, Hui Yan¹, Xiang Xiao¹, Dawei Zou¹, Xiaolong Zhang¹, Xiongbing Zu⁵, Xian C Li^{1

6}, Wenhao Chen^{1

6}

Affiliations

¹ Immunobiology and Transplant Science Center, Department of Surgery, Houston Methodist Research Institute and Institute for Academic Medicine, Houston Methodist Hospital, Houston, TX, USA.
² Department of Urology, First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, China.
³ Systems Medicine and Bioengineering Department, Houston Methodist Neal Cancer Center, Houston, TX, USA.
⁴ Department of Radiology, Houston Methodist Hospital, Weill Cornell Medicine, Houston, TX, USA.
⁵ Department of Urology, Xiangya Hospital, Central South University, Changsha, Hunan, China.
⁶ Department of Surgery, Weill Cornell Medicine, Cornell University, New York, NY, USA.

Abstract

T-cell-based immunotherapy is gaining momentum in cancer treatment; however, our comprehension of the transcriptional regulation governing T cell antitumor activity remains constrained. The objective of this study was to explore the function of interferon regulatory factor 4 (IRF4) in antitumor CD8⁺ T cells using the TRAMP-C1 prostate cancer and B16F10 melanoma model. To achieve this, we generated an Irf4^GFP-DTR mouse strain and discovered that CD8⁺ tumor-infiltrating lymphocytes (TILs) expressing high levels of IRF4.GFP exhibited a more differentiated PD-1^high cell phenotype. By administering diphtheria toxin to tumor-bearing Irf4^GFP-DTR mice, we partially depleted IRF4.GFP⁺ TILs and observed an accelerated tumor growth. To specifically explore the function of IRF4 in antitumor CD8⁺ T cells, we conducted 3 adoptive cell therapy (ACT) models. Firstly, depleting IRF4.GFP⁺ CD8⁺ TILs derived from ACT significantly accelerated tumor growth, emphasizing their crucial role in controlling tumor progression. Secondly, deleting the Irf4 gene in antitumor CD8⁺ T cells used for ACT led to a reduction in the frequency and effector differentiation of CD8⁺ TILs, completely abolishing the antitumor effects of ACT. Lastly, we performed a temporal deletion of the Irf4 gene in antitumor CD8⁺ T cells during ACT, starting from 20 days after tumor implantation, which significantly compromised tumor control. Therefore, sustained expression of IRF4 is essential for maintaining CD8⁺ T cell immunity in the melanoma model, and these findings carry noteworthy implications for the advancement of more potent immunotherapies for solid tumors.