Transcriptome Sequencing of Gingival Tissues from Impacted Third Molars Patients Reveals the Alterations of Gene Expression

Haolin Zhou; Nanqing Fu; Yuan Tian; Nini Zhang; Qin Fan; Fengjiao Zeng; Yueyue Wang; Guohui Bai; Bin Chen

doi:10.2174/0113862073256803231114095626

Transcriptome Sequencing of Gingival Tissues from Impacted Third Molars Patients Reveals the Alterations of Gene Expression

Comb Chem High Throughput Screen. 2024 Jan 4. doi: 10.2174/0113862073256803231114095626. Online ahead of print.

Authors

Haolin Zhou^{1

2}, Nanqing Fu¹, Yuan Tian^{1

2}, Nini Zhang², Qin Fan^{1

2}, Fengjiao Zeng^{2

1}, Yueyue Wang^{1

2}, Guohui Bai^{1

2}, Bin Chen¹

Affiliations

¹ Oral Disease Research Key Laboratory of Guizhou Tertiary Institution, Institute of Life Sciences, Zunyi Medical University, Zunyi, Guizhou 563000, China.
² Hospital of Stomatology, Zunyi Medical University, Zunyi, Guizhou 563000, China.

PMID: 38178683
DOI: 10.2174/0113862073256803231114095626

Abstract

Objective: The removal of impacted third molars by surgery may occur with a series of complications, whereas limited information about the postoperative pathogenesis is available. The objective of this study is to identify changes in gene expression after flap surgical removal of impacted third molars and provide potential information to reduce postoperative complications.

Methods: The gingival tissues of twenty patients with flap surgical removal of impacted third molars and twenty healthy volunteers were collected for gene expression testing. The collected gingival tissues were used RNA sequencing technology and quantitative real-time PCR validation was performed. DEG was mapped to protein databases such as GO and KEGG for functional annotation and, based on annotation information, for mining of differential expression genes in patients with mpacted third molars.

Results: A total of 555 genes were differentially expressed. Among the top up-regulated genes, HLA-DRB4, CCL20, and CXCL8 were strongly associated with immune response and signal transduction. Among the top down-regulated genes, SPRR2B, CLDN17, LCE3D and LCE3E were related to keratinocyte differentiation, IFITM5, and BGLAP were related to bone mineralization, UGT2B17 is associated with susceptibility to osteoporosis. KEGG results showed that the DEGs were related to multiple disease-related pathways.

Conclusion: This first transcriptome analysis of gingival tissues from patients with surgical removal of impacted third molars provides new insights into postoperative genetic changes. The results may establish a basis for future research on minimizing the incidence of complications after flap-treated third molars.

Keywords: . impacted third molar; RNA-sequencing; differentially expressed genes; flap surgery; gingival tissue; transcriptome.