Lipid hydrolysis monitoring, including especially glycerides, is necessary for comprehending the anaerobic digestion process in lipid-rich substrates processing. This reaction has not been investigated in such detail so far, despite its potential to be crucial in assuring a stable process. This study suggested and thoroughly validated an uncomplicated method of monitoring lipid hydrolysis during anaerobic digestion, achieving recovery values >95 % with an average relative standard deviation <5 %. Subsequently, the method was applied on the very first detailed observation of glyceride hydrolysis in the anaerobic sludge, tracking even changes in fatty acid profiles during anaerobic digestion. Results showed that lipid hydrolysis can take several days, thus likely affecting the whole anaerobic digestion of lipids. The method aims to provide answers to improve understanding of lipids' fate and their inhibition phenomena in anaerobic digestion.
Keywords: Glyceride; Lipid-rich substrate; Long-chain fatty acids; Sludge; Validation.
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