Preparation and application of caffeic acid imprinted polymer

Şeyda Karaman Ersoy; Esma Tütem; Kevser Sözgen Başkan; Reşat Apak

doi:10.55730/1300-0527.3572

Preparation and application of caffeic acid imprinted polymer

Turk J Chem. 2023 May 22;47(4):699-714. doi: 10.55730/1300-0527.3572. eCollection 2023.

Authors

Şeyda Karaman Ersoy¹, Esma Tütem², Kevser Sözgen Başkan², Reşat Apak^{2

3}

Affiliations

¹ Division of Analytical Chemistry, Faculty of Pharmacy, Fenerbahçe University, İstanbul, Turkiye.
² Department of Chemistry, Faculty of Engineering, İstanbul University-Cerrahpaşa, İstanbul, Turkiye.
³ Turkish Academy of Sciences (TÜBA), Ankara, Turkiye.

Abstract

In the present study, molecularly imprinted polymers were synthesized using caffeic acid (CA) as a template molecule and then used for the extraction of CA and chlorogenic acid (CLA) from complex matrices. Syntheses were carried out in tetrahydrofuran as porogenic solvent using 4-vinyl pyridine, methacrylic acid, acrylamide, and 1-vinyl imidazole as monomers, ethylene glycol dimethacrylate as crosslinker and 2,2'-azobisisobutyronitrile as initiator. In polymerization processes, different ratios of the template:monomer:crosslinker (T:M:CrL) were used to obtain the most suitable polymer. Caffeic acid:4-vinylpiridine:ethylene glycol dimethacrylate's 1:4:16 mole ratio of MIP was determined as the most convenient polymer for CA recognition. In addition, nonimprinted polymers (NIPs) without templates were prepared. Dynamic and static adsorption tests were applied to determine the absorption features of the NIPs and CA-MIPs. Separation and purification studies of CA and CLA were performed with molecular imprinted solid phase extraction (MISPE) application. All steps of MISPE (loading, washing, elution) were optimized by HPLC analysis.

Keywords: Caffeic acid imprinted polymer; caffeic acid recovery; chlorogenic acid recovery; green coffee bean; molecularly imprinted solid phase extraction.