Analysis of associations between the TLR3 SNPs rs3775291 and rs3775290 and COVID-19 in a cohort of professionals of Belém-PA, Brazil

Marcos Jessé Abrahão Silva; Marcelo Cleyton da Silva Vieira; Alex Brito Souza; Everaldina Cordeiro Dos Santos; Beatriz Dos Reis Marcelino; Samir Mansour Moraes Casseb; Karla Valéria Batista Lima; Luana Nepomuceno Gondim Costa Lima

doi:10.3389/fcimb.2023.1320701

Analysis of associations between the TLR3 SNPs rs3775291 and rs3775290 and COVID-19 in a cohort of professionals of Belém-PA, Brazil

Front Cell Infect Microbiol. 2023 Dec 19:13:1320701. doi: 10.3389/fcimb.2023.1320701. eCollection 2023.

Authors

Marcos Jessé Abrahão Silva¹, Marcelo Cleyton da Silva Vieira¹, Alex Brito Souza¹, Everaldina Cordeiro Dos Santos¹, Beatriz Dos Reis Marcelino¹, Samir Mansour Moraes Casseb², Karla Valéria Batista Lima¹, Luana Nepomuceno Gondim Costa Lima¹

Affiliations

¹ Molecular Biology Laboratory, Bacteriology and Mycology Section (SABMI), Evandro Chagas Institute (IEC), Ananindeua, Brazil.
² Molecular Biology Laboratory, Pathology Section (SEPAT), Evandro Chagas Institute (IEC), Ananindeua, Brazil.

Abstract

The objective of this article was to verify associations between the SNPs rs3775291 (Cytosine [C]>Thymine [T]) and rs3775290 (C>T) of TLR3 in professionals from Health Institutions (HI) who worked during the first pandemic wave and COVID-19. A case-control study was carried out with workers from HI in Belém-PA, Brazil, divided into symptomatology groups (Asymptomatic-AS, n=91; and Symptomatic-SI, n=121), and severity groups, classified by Chest CT scan (symptomatic with lung involvement - SCP, n=34; symptomatic without lung involvement - SSP, n=8). Genotyping was performed by Sanger sequencing and statistical analysis was performed using the SPSS program. In the analysis of SNP rs3775291, the homozygous recessive genotype (T/T) was not found and the frequency of the mutant allele (T) was less than 2% in the cohort. For the rs3775290 SNP, the frequency of the mutant allele (T) was greater than 42% in the cohort. No significant associations were found for these SNPs in this cohort (N= 212 individuals). The scientific community and physicians can use these facts to find new methods of managing COVID-19.

Keywords: COVID-19; TLR3; epidemiology; genetics; single nucleotide polymorphism.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Brazil / epidemiology
COVID-19* / genetics
Case-Control Studies
Genetic Predisposition to Disease
Genotype
Humans
Polymorphism, Single Nucleotide*
Toll-Like Receptor 3 / genetics

Substances

Toll-Like Receptor 3
TLR3 protein, human

Grants and funding

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. MS was financed by the CAPES Grant – Financial Code 88887.888066/2023-00 and APC was financed by the Evandro Chagas Institute (IEC).