Glucose, essential for brain and muscle functions, requires careful monitoring in diabetes and other chronic disease management. While blood glucose monitoring provides precise information about these diseases, it remains an invasive method. Saliva glucose monitoring could offer an alternative approach, but the glucose concentration in saliva is very low. In this work, we report a simple, low-cost, highly sensitive nonenzymatic electrochemical glucose sensor. We developed this sensor using green synthesized gold nanoparticles (AuNPs) and wet chemical synthesized copper oxide (CuO) nanoparticles on a screen-printed carbon electrode (Au/CuO/SPCE). The sensor's high sensitivity results from dual amplification strategies using AuNPs and CuO nanomaterials, each demonstrating catalytic activity towards glucose. This shows promising potential for saliva glucose monitoring. The AuNPs were synthesized using an Au precursor and orange peel extract (OPE), yielding stable colloidal AuNPs with a mean diameter of about 37 nm, thus eliminating the need for additional capping agents. Under optimal conditions, amperometric tests revealed that the sensor responded linearly to glucose concentrations ranging from 2 μM to 397 μM with a sensitivity of 236.70 μA mM-1 cm-2. Furthermore, the sensor demonstrated excellent reproducibility, stability and high selectivity for glucose in the presence of different biomolecules. We validated the sensor's efficacy by measuring glucose in human saliva, showing its potential for noninvasive glucose monitoring. This research advances the development of point-of-care devices, positioning the sensor as a promising tool for noninvasive glucose monitoring and improved diabetes management.
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