Applications of CRISPR screening to lung cancer treatment

Wanying Shen; Fangli Hu; Pan Lei; Yijun Tang

doi:10.3389/fcell.2023.1295555

Applications of CRISPR screening to lung cancer treatment

Front Cell Dev Biol. 2023 Dec 15:11:1295555. doi: 10.3389/fcell.2023.1295555. eCollection 2023.

Authors

Wanying Shen^#^{1

2}, Fangli Hu^#², Pan Lei^{2

3}, Yijun Tang^{1

2}

Affiliations

¹ Department of Neurosurgery, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, China.
² Hubei Hongshan Laboratory, College of Biomedicine and Health, Huazhong Agricultural University, Wuhan, China.
³ Hubei Clinical Research Center for Precise Diagnosis and Treatment of Liver Cancer, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, China.

^# Contributed equally.

Abstract

Lung cancer is an extremely aggressive and highly prevalent disease worldwide, and it is one of the leading causes of cancer death. Deciphering intrinsic genetic mechanism, finding new targets, and overcoming drug resistance are the key to lung cancer treatment. High-throughput CRISPR screening has been extensively used to obtain the genes related to cancers including lung cancer. This review describes CRISPR/Cas9 or CRISPR/dCas9-based technologies for high-throughput screening. We summarize the applications of CRISPR screening technology in exploring the mechanism of lung cancer development in vivo or in vitro, overcoming drug resistance, improving the effect of immunotherapy, and discovering new therapeutic targets. This review highlights the potential of CRISPR screening in combination with tumor barcoding and high-throughput sequencing (Tuba-seq) to precisely quantify the impact of alterations in many tumor suppressor genes on lung cancer.

Keywords: CRISPR screening; Tuba-seq; drug resistance; lung cancer; treatment.

Publication types

Review

Grants and funding

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. PL is supported by the Science and Technology Research Project of Hubei Education Department (No. B2019110); YT is supported by the Hubei Province medical leading talents training project (No. 201947).