Elucidating the efficacy of functionalized multi-walled carbon nanotube in the biogenesis of L-Dopa and antioxidant metabolites in cell cultures of Hybanthus enneaspermus

Shanthi Pandurengan Parthasarathy; Sathiyanarayanan Anusuya; Subramaniyan Rajalakshmi; Davis Megha; Chinnaswamy Appunu; Subramaniyam Alagumanian; Markandan Manickavasagam

doi:10.1016/j.plaphy.2023.108310

Elucidating the efficacy of functionalized multi-walled carbon nanotube in the biogenesis of L-Dopa and antioxidant metabolites in cell cultures of Hybanthus enneaspermus

Plant Physiol Biochem. 2024 Jan:206:108310. doi: 10.1016/j.plaphy.2023.108310. Epub 2023 Dec 29.

Authors

Shanthi Pandurengan Parthasarathy¹, Sathiyanarayanan Anusuya², Subramaniyan Rajalakshmi¹, Davis Megha¹, Chinnaswamy Appunu³, Subramaniyam Alagumanian⁴, Markandan Manickavasagam⁵

Affiliations

¹ Department of Biotechnology, School of Biotechnology and Genetic Engineering, Bharathidasan University, Tiruchirappalli, 620024, Tamilnadu, India.
² Department of Botany, St. Joseph's College (Autonomous), Tiruchirappalli, 620002, Tamilnadu, India.
³ Division of Crop Improvement, Sugarcane Breeding Institute, (ICAR), Coimbatore, 641007, Tamilnadu, India.
⁴ Department of Botany, H.H. The Rajah's College (Autonomous), Pudukkottai, 622001, Tamilnadu, India.
⁵ Department of Biotechnology, School of Biotechnology and Genetic Engineering, Bharathidasan University, Tiruchirappalli, 620024, Tamilnadu, India. Electronic address: manickbiotech@gmail.com.

PMID: 38169226
DOI: 10.1016/j.plaphy.2023.108310

Abstract

Hybanthus enneaspermus (L.)F.Muell. is a highly indispensable medicinal herb yielding L-Dopa, deemed the gold standard drug among the therapeutic options for Parkinson's disease. This investigation is the first attempt to evaluate the eliciting influence of carboxylic acid functionalized multi-walled carbon nanotube (MWCNT-COOH) on the biosynthesis of L-Dopa and on biomass aggregation and antioxidant metabolites in H. enneaspermus cell suspension cultures. Suspension cells were accomplished from friable calli generated from the nodal segments of H. enneaspermus in Murashige and Skoog (MS) liquid medium infused with 2 mg L^-1 2, 4-Dichlorophenoxyacetic acid (2, 4-D), and 0.3 mg L^-1meta-Topolin (mT). The influence of MWCNTs on L-Dopa synthesis, biomass accumulation, and biochemical parameters was examined on the basis of the exposure time and in a concentration-dependent manner of MWCNTs. The inclusion of 30 mg L^-1 MWCNTs increased the biomass and the L-Dopa level by 2.00 and 16.37-folds, respectively, compared with that of the control. Furthermore, the effect of MWCNTs on physiological parameters such as catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPX), ascorbate peroxidase (APX), hydrogen peroxide (H₂O₂), malondialdehyde (MDA) content, 2-diphenylpicrylhydrazyl (DPPH), and ferric-reducing ability of plasma (FRAP) was examined over the elicited cells. Among the antioxidant enzymatic activities, CAT enhanced 8.0 fold compared with that of the control. MDA and DPPH content enhanced 2.60 and 1.12 folds, respectively, compared with that of the control. The current study showed that MWCNTs offer new possibilities for their usage over in vitro by acting as potential innovative plant metabolite elicitors and stress-protecting entities.

Keywords: Antioxidant metabolites; Cell suspension cultures; Growth kinetics; L-Dopa. Hybanthus enneaspermus; Multi-walled carbon nanotubes.

MeSH terms

Antioxidants / metabolism
Hydrogen Peroxide / metabolism
Levodopa
Nanotubes, Carbon*
Violaceae* / chemistry
Violaceae* / metabolism

Substances

Antioxidants
Nanotubes, Carbon
Levodopa
Hydrogen Peroxide