Role of plant metabolites in the formation of bacterial communities in the rhizosphere of Tetrastigma hemsleyanum

Yuqing Huang; Hongliang Hu; Erkui Yue; Wu Ying; Tianxin Niu; Jianli Yan; Qiujun Lu; Songlin Ruan

doi:10.3389/fmicb.2023.1292896

Role of plant metabolites in the formation of bacterial communities in the rhizosphere of Tetrastigma hemsleyanum

Front Microbiol. 2023 Dec 15:14:1292896. doi: 10.3389/fmicb.2023.1292896. eCollection 2023.

Authors

Yuqing Huang¹, Hongliang Hu², Erkui Yue¹, Wu Ying¹, Tianxin Niu¹, Jianli Yan¹, Qiujun Lu³, Songlin Ruan¹

Affiliations

¹ Institute of Crop Science, Hangzhou Academy of Agricultural Sciences, Hangzhou, China.
² College of Life Sciences, China Jiliang University, Hangzhou, China.
³ Hangzhou Agricultural and Rural Affairs Guarantee Center, Hangzhou, China.

Abstract

Tetrastigma hemsleyanum Diels et Gilg, commonly known as Sanyeqing (SYQ), is an important traditional Chinese medicine. The content of bioactive constituents varies in different cultivars of SYQ. In the plant growth related researches, rhizosphere microbiome has gained significant attention. However, the role of bacterial communities in the accumulation of metabolites in plants have not been investigated. Herein, the composition of bacterial communities in the rhizosphere soils and the metabolites profile of different SYQ cultivars' roots were analyzed. It was found that the composition of microbial communities varied in the rhizosphere soils of different SYQ cultivars. The high abundance of Actinomadura, Streptomyces and other bacteria was found to be associated with the metabolites profile of SYQ roots. The findings suggest that the upregulation of rutin and hesperetin may contribute to the high bioactive constituent in SYQ roots. These results provide better understanding of the metabolite accumulation pattern in SYQ, and also provide a solution for enhancing the quality of SYQ by application of suitable microbial consortia.

Keywords: Sanyeqing; Tetrastigma hemsleyanum; bioactive constituents; metabolite profiling; metagenome; microbial community structure; rhizosphere microbiome.

Grants and funding

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This study was supported by Zhejiang Province Traditional Chinese Medicine Industry Technology Project (202210), Hangzhou Agricultural Science and Technology Cooperation and Innovation (202209TD19) and Science and Technology Innovation and Demonstration extension Fund of Hangzhou Academy of Agricultural Sciences (no. 2022HNCT-07).