Periodontitis relates to benign prostatic hyperplasia via the gut microbiota and fecal metabolome

Xing-Pei Guo; Jun Yang; Lan Wu; Cheng Fang; Jia-Min Gu; Fei Li; Han-Song Liu; Lu-Yao Li; Shuang-Ying Wang

doi:10.3389/fmicb.2023.1280628

Periodontitis relates to benign prostatic hyperplasia via the gut microbiota and fecal metabolome

Front Microbiol. 2023 Dec 13:14:1280628. doi: 10.3389/fmicb.2023.1280628. eCollection 2023.

Authors

Xing-Pei Guo^#^{1

2}, Jun Yang^#³, Lan Wu^{2

4}, Cheng Fang², Jia-Min Gu^{2

5}, Fei Li^{2

5}, Han-Song Liu¹, Lu-Yao Li^{2

5}, Shuang-Ying Wang²

Affiliations

¹ Department of General Surgery, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou, Henan, China.
² Center for Evidence-Based and Translational Medicine, Zhongnan Hospital of Wuhan University, Wuhan, China.
³ Department of Urology, The First People's Hospital of Tianmen in Hubei Province, The Affiliated Hospital of Hubei University of Science and Technology, Tianmen, China.
⁴ Department of Stomatology, Zhongnan Hospital of Wuhan University, Wuhan, China.
⁵ Department of Urology, Zhongnan Hospital of Wuhan University, Wuhan, China.

^# Contributed equally.

Abstract

Objectives: Periodontitis is associated with benign prostatic hyperplasia (BPH), whether it related to gut floramicrobiota and metabonomics is unclear.

Methods: We established ligature-induced periodontitis (EP), testosterone-induced BPH, and composite rat models. Fecal samples were collected to detect gut microbiota by 16S rDNA sequencing and metabonomics were detected by liquid chromatography tandem mass spectrometry (LC-MS/MS).

Results: Sequencing results revealed differential gut floramicrobiota composition between EP+BPH group and other three groups. The abundances of Ruminococcus flavefaciens were significantly increased in EP+BPH group compared with other groups. Tenericutes, Mollicutes, RF39 and Ruminococcus gnavus were significantly decreased in EP+BPH group compared with BPH group, while Ruminococcus callidus and Escherichia were significantly decreased compared with EP group. For gut metabonomics, LC-MS/MS showed that fecal metabolites and seven metabolic pathways were changed in EP+BPH group, such as biosynthesis of unsaturated fatty acids, steroid hormone biosynthesis. Correlation analysis showed that the alterations of gut metabolism were significantly correlated with differential gut floramicrobiota, such as Ruminococcus callidus and Ruminococcus flavefaciens.

Conclusion: Our study highlights the relationship of periodontitis and BPH, the alterations of gut floramicrobiota and metabolites may be involved in two diseases, which provides new idea for prevention and treatment of patients with periodontitis concurrent BPH.

Keywords: benign prostatic hyperplasia; fecal metabolites; gut microbiota; multi-omics analysis; periodontitis.

Grants and funding

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This study was supported (in part) by the Fundamental Research Funds for the Central Universities (2042022kf0072, 2042021kf1041, 2042020kf0130) and the Nature Science Foundation of Hubei Province (2019CFB760).