Functionalized biomimetic mineralized collagen promotes osseointegration of 3D-printed titanium alloy microporous interface

Xiao Sheng; He Liu; Yu Xu; Zhonghan Wang; Weimin Zhang; Chen Li; Jincheng Wang

doi:10.1016/j.mtbio.2023.100896

Functionalized biomimetic mineralized collagen promotes osseointegration of 3D-printed titanium alloy microporous interface

Mater Today Bio. 2023 Dec 5:24:100896. doi: 10.1016/j.mtbio.2023.100896. eCollection 2024 Feb.

Authors

Xiao Sheng¹, He Liu^{1

2}, Yu Xu³, Zhonghan Wang^{1

2}, Weimin Zhang¹, Chen Li^{1

2}, Jincheng Wang^{1

2}

Affiliations

¹ Department of Orthopedics, The Second Hospital of Jilin University, Changchun, 130041, Jilin, China.
² Orthopaedic Research Institute of Jilin Province, Changchun, 130041, China.
³ Department of Ophthalmologic, The Second Hospital of Jilin University, Changchun, 130041, Jilin, China.

Abstract

Mineralized collagen (MC) is the fundamental unit of natural bone tissue and can induce bone regeneration. Unmodified MC has poor mechanical properties and a single component, making it unable to cope with complex physiological environment. In this study, we introduced sodium alginate (SA) and vascular endothelial growth factor (VEGF) into the MC material to construct functionalized mineralized collagen (FMC) with good mechanical strength and the ability to continuously release growth factors. The FMC is filled into the pores of 3D printed titanium alloy scaffold to form a new organic-inorganic bioactive interface. With the continuous degradation of FMC, bone marrow mesenchymal stem cells (BMSCs) and vascular endothelial cells (VECs) in the surrounding environment are recruited to the surface of the scaffold to promote bone and vascular regeneration. After implanting the scaffold into the distal femoral defect of rabbits, Micro CT, histological, push-out, as well as immunohistochemical analysis showed that the composite interface can significantly promote osseointegration. These findings provide a new strategy for the development and application of mineralized collagen materials.

Keywords: 3D-printed porous titanium scaffold; Angiogenesis; Bionic interface; Growth factor; Mineralized collagen; Osseointegration.