Hellebrigenin triggers death of promyelocytic leukemia cells by non-genotoxic ways

Toxicon. 2024 Feb 1:238:107591. doi: 10.1016/j.toxicon.2023.107591. Epub 2023 Dec 29.

Abstract

Bufadienolides are digitalis-like aglycones mainly found in skin secretions of toads. Among their biological properties, the mechanisms of antiproliferative action on tumor cells remain unclear for many compounds, including against leukemia cells. Herein, it was evaluated the mechanisms involved in the antiproliferative and genotoxic actions of hellebrigenin on tumor cell lines and in silico capacity to inhibit the human topoisomerase IIa enzyme. Firstly, its cytotoxic action was investigated by colorimetric assays in human tumor and peripheral blood mononuclear cells (PBMC). Next, biochemical and morphological studies were detailed by light microscopy (trypan blue dye exclusion), immunocytochemistry (BrdU uptake), flow cytometry and DNA/chromosomal damages (Cometa and aberrations). Finally, computational modelling was used to search for topoisomerase inhibition. Hellebrigenin reduced proliferation, BrdU incorporation, viability, and membrane integrity of HL-60 leukemia cells. Additionally, it increased G2/M arrest, internucleosomal DNA fragmentation, mitochondrial depolarization, and phosphatidylserine externalization in a concentration-dependent manner. In contrast to doxorubicin, hellebrigenin did not cause DNA strand breaks in HL-60 cell line and lymphocytes, and it interacts with ATPase domain residues of human topoisomerase IIa, generating a complex of hydrophobic and van der Waals interactions and hydrogen bonds. So, hellebrigenin presented potent anti-leukemic activity at concentrations as low as 0.06 μM, a value comparable to the clinical anticancer agent doxorubicin, and caused biochemical changes suggestive of apoptosis without genotoxic/clastogenic-related action, but it probably triggers catalytic inhibition of topoisomerase II. These findings also emphasize toad steroid toxins as promising lead antineoplasic compounds with relatively low cytotoxic action on human normal cells.

Keywords: Antitumor poison; Apoptosis; Bufadienolide; Cell cycle arrest; Topoisomerase inhibitor.

MeSH terms

  • Antineoplastic Agents* / pharmacology
  • Apoptosis
  • Bromodeoxyuridine / pharmacology
  • Bufanolides* / chemistry
  • DNA / pharmacology
  • DNA Damage
  • Doxorubicin / pharmacology
  • HL-60 Cells
  • Humans
  • Leukemia*
  • Leukocytes, Mononuclear

Substances

  • hellebrigenin
  • Bromodeoxyuridine
  • Antineoplastic Agents
  • Bufanolides
  • DNA
  • Doxorubicin