Characterization and anti-tumor activities of polysaccharide isolated from Brassica rapa L. via activation of macrophages through TLR2-and TLR4-Dependent pathways

Rina Matsui; Katsunori Endo; Takeru Saiki; Hazuki Haga; Weidong Shen; Xiangdong Wang; Shinya Yamazaki; Shigeru Katayama; Kenji Nagata; Hidemitsu Kitamura; Sachi Tanaka

doi:10.1016/j.abb.2023.109879

Characterization and anti-tumor activities of polysaccharide isolated from Brassica rapa L. via activation of macrophages through TLR2-and TLR4-Dependent pathways

Arch Biochem Biophys. 2024 Feb:752:109879. doi: 10.1016/j.abb.2023.109879. Epub 2023 Dec 29.

Authors

Affiliations

¹ Division of Food Science and Biotechnology, Department of Agriculture, Graduate School of Science and Technology, Shinshu University, Minami-minowa, Kami-ina, Nagano, 399-4598, Japan.
² Division of Food Science and Biotechnology, Department of Science and Technology Agriculture, Graduate School of Medicine, Science and Technology, Shinshu University, Minami-minowa, Kami-ina, Nagano, 399-4598, Japan.
³ Division of Functional Immunology, Section of Disease Control, Institute for Genetic Medicine, Hokkaido University, Kita-15, Nishi-7, Kita-Ku, Sapporo, Hokkaido, 090-0815, Japan.
⁴ Food Technology Department, Nagano Prefecture General Industrial Technology Center, 205-1 Kurita, Nagano, Nagano, 380-0921, Japan.
⁵ Division of Food Science and Biotechnology, Department of Agriculture, Graduate School of Science and Technology, Shinshu University, Minami-minowa, Kami-ina, Nagano, 399-4598, Japan; Division of Food Science and Biotechnology, Department of Science and Technology Agriculture, Graduate School of Medicine, Science and Technology, Shinshu University, Minami-minowa, Kami-ina, Nagano, 399-4598, Japan.
⁶ Department of Life Science and Applied Chemistry, Graduate School of Engineering, Nagoya Institute of Technology, Gokiso-cho, Showa-ku, Nagoya, Aichi, 466-8555, Japan.
⁷ Division of Functional Immunology, Section of Disease Control, Institute for Genetic Medicine, Hokkaido University, Kita-15, Nishi-7, Kita-Ku, Sapporo, Hokkaido, 090-0815, Japan; Department of Biomedical Engineering, Faculty of Science and Engineering, Toyo University, Kawagoe, Saitama, 350-8585, Japan.
⁸ Division of Food Science and Biotechnology, Department of Agriculture, Graduate School of Science and Technology, Shinshu University, Minami-minowa, Kami-ina, Nagano, 399-4598, Japan; Division of Food Science and Biotechnology, Department of Science and Technology Agriculture, Graduate School of Medicine, Science and Technology, Shinshu University, Minami-minowa, Kami-ina, Nagano, 399-4598, Japan. Electronic address: tanakasa@shinshu-u.ac.jp.

PMID: 38160699
DOI: 10.1016/j.abb.2023.109879

Abstract

We have previously shown the immunostimulatory effects by Nozawana (Brassica rapa L.). In this report, we determined the characteristics of Nozawana polysaccharide (NPS) and evaluated the immunomodulatory effects and anti-tumor activity of NPS mediated by macrophage activation. The molecular weight of NPS was determined by gel filtration chromatography with an average molecular weight of approximately 100.6 kDa. HPLC analysis showed that NPS contained glucose, galacturonic acid, galactose, and arabinose. NPS increased cytokine and nitric oxide (NO) production by macrophages in a Toll-like receptor (TLR)2 and TLR4-dependent manner. Furthermore, NPS induced apoptosis significantly against 4T1 murine breast cancer cells cultured in conditioned medium from NPS-treated macrophages through tumor necrosis factor-α. In tumor-bearing mouse model, tumor growth was significantly reduced in NPS-treated mice compared with control mice. These results support the potential use of NPS as an immunotherapeutic material found in health food products.

Keywords: Anti-tumor activity; Brassica rapa L.; Nozawana polysaccharide; Toll-like receptor.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Brassica rapa* / metabolism
Macrophage Activation
Macrophages / metabolism
Mice
Polysaccharides / metabolism
Polysaccharides / pharmacology
Toll-Like Receptor 2* / metabolism
Toll-Like Receptor 4 / metabolism
Tumor Necrosis Factor-alpha / metabolism

Substances

Toll-Like Receptor 2
Toll-Like Receptor 4
Polysaccharides
Tumor Necrosis Factor-alpha