Strategies for the biological production of ectoine by using different chassis strains

Yifan Feng; Min Qiu; Lei Shao; Yujia Jiang; Wenming Zhang; Wankui Jiang; Fengxue Xin; Min Jiang

doi:10.1016/j.biotechadv.2023.108306

Strategies for the biological production of ectoine by using different chassis strains

Biotechnol Adv. 2024 Jan-Feb:70:108306. doi: 10.1016/j.biotechadv.2023.108306. Epub 2023 Dec 28.

Authors

Yifan Feng¹, Min Qiu¹, Lei Shao¹, Yujia Jiang², Wenming Zhang², Wankui Jiang³, Fengxue Xin⁴, Min Jiang²

Affiliations

¹ State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, PR China.
² State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, PR China; Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing Tech University, Nanjing 211816, PR China.
³ State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, PR China. Electronic address: jiangwankui@njtech.edu.cn.
⁴ State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, PR China; Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing Tech University, Nanjing 211816, PR China. Electronic address: xinfengxue@njtech.edu.cn.

PMID: 38157997
DOI: 10.1016/j.biotechadv.2023.108306

Abstract

As an amino acid derivative and a typical compatible solute, ectoine can assist microorganisms in resisting high osmotic pressure. Own to its long-term moisturizing effects, ectoine shows extensive applications in cosmetics, medicine and other fields. With the rapid development of synthetic biology and fermentation engineering, many biological strategies have been developed to improve the ectoine production and simplify the production process. Currently, the microbial fermentation has been widely used for large scaling ectoine production. Accordingly, this review will introduce the metabolic pathway for ectoine synthesis and also comprehensively evaluate both wild-type and genetically modified strains for ectoine production. Furthermore, process parameters affecting the ectoine production efficiency and adoption of low cost substrates will be evaluated. Lastly, future prospects on the improvement of ectoine production will be proposed.

Keywords: Biosynthesis; Ectoine; Genetic engineering; Metabolic engineering.

Publication types

Review
Research Support, Non-U.S. Gov't

MeSH terms

Amino Acids, Diamino* / chemistry
Amino Acids, Diamino* / metabolism
Fermentation
Metabolic Networks and Pathways

Substances

ectoine
Amino Acids, Diamino