Simultaneous detection of norepinephrine and 5-hydroxytryptophan using poly-alizarin/multi-walled carbon nanotubes-graphene modified carbon fiber microelectrode array sensor

Chaoying Zeng; Yulan Li; Mingfang Zhu; Zengcheng Du; Huanru Liang; Qiqing Chen; Hongqing Ye; Rui Li; Wenhao Liu

doi:10.1016/j.talanta.2023.125565

Simultaneous detection of norepinephrine and 5-hydroxytryptophan using poly-alizarin/multi-walled carbon nanotubes-graphene modified carbon fiber microelectrode array sensor

Talanta. 2024 Apr 1:270:125565. doi: 10.1016/j.talanta.2023.125565. Epub 2023 Dec 20.

Authors

Chaoying Zeng¹, Yulan Li², Mingfang Zhu³, Zengcheng Du², Huanru Liang², Qiqing Chen², Hongqing Ye², Rui Li², Wenhao Liu²

Affiliations

¹ College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou Higher Education Mega Center, Guangzhou, 510006, PR China. Electronic address: 843017341@qq.com.
² College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou Higher Education Mega Center, Guangzhou, 510006, PR China.
³ College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou Higher Education Mega Center, Guangzhou, 510006, PR China. Electronic address: zhumfgy@126.com.

PMID: 38154355
DOI: 10.1016/j.talanta.2023.125565

Abstract

Multi-walled carbon nanotubes, graphene and alizarin polymer composites coated carbon fiber microelectrode array sensor (p-AZ/MWCNT-GR/CFMEA) was constructed and used for the simultaneous detection of norepinephrine (NE) and 5-hydroxytryptophan (5-HT). The morphology and structural characteristics of sensor are characterized using scanning electron microscopy, energy dispersive spectroscopy and X-ray diffraction. Its electrochemical behavior has been studied with cyclic voltammetry and electrochemical impedance spectroscopy. The sensor exhibits excellent electrochemical activity for the oxidation of NE and 5-HT, two well separated oxidation peaks with the peak potential difference of 220 mV are observed on the cyclic voltammogram. NE and 5-HT both show two electrons and two protons electrochemical reaction on the p-AZ/MWCNT-GR/CFMEA. Under the optimized experiment conditions, the linear ranges of the sensor for NE and 5-HT are 0. 08- 8 μM and 0. 1-20 μM with detection limits of 4. 22 nM and 14. 2 nM (S/N = 3), respectively. In addition, the microsensor array show good reproducibility, stability and selectivity for the determination of NE and 5-HT. Finally, the p-AZ/MWCNT-GR/CFMEA is applied to the simultaneous detection of NE and 5-HT in human serum samples and macrophages.

Keywords: 5-Hydroxytryptophan; Carbon fiber microelectrode array; MWCNT-GR hybrid; Norepinephrine; Poly-alizarin.