Bioinspired Zn-MOF doped radial porous chitosan-based sponge with antibacterial and antioxidant properties for rapid hemostasis and wound healing

Qingwu Wang; Qiaoyi Han; Xin Xu; Xu Ding; Xiaojuan Nie; Xiaodong Xu; Xia Liu; Chunhong Zhang; Junqing Li; Qiang Shi

doi:10.1016/j.ijbiomac.2023.128960

Bioinspired Zn-MOF doped radial porous chitosan-based sponge with antibacterial and antioxidant properties for rapid hemostasis and wound healing

Int J Biol Macromol. 2024 Feb;259(Pt 2):128960. doi: 10.1016/j.ijbiomac.2023.128960. Epub 2023 Dec 25.

Authors

Qingwu Wang¹, Qiaoyi Han², Xin Xu¹, Xu Ding¹, Xiaojuan Nie¹, Xiaodong Xu³, Xia Liu⁴, Chunhong Zhang⁵, Junqing Li¹, Qiang Shi⁶

Affiliations

¹ Key Laboratory of Superlight Materials & Surface Technology, Ministry of Education, College of Materials Science and Chemical Engineering, Harbin Engineering University, Harbin 150001, PR China.
² State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China.
³ Key Laboratory of Superlight Materials & Surface Technology, Ministry of Education, College of Materials Science and Chemical Engineering, Harbin Engineering University, Harbin 150001, PR China. Electronic address: xuxiaodong@hrbeu.edu.cn.
⁴ School of Chemstry, Southwest Jiaotong University, Chengdu 610031, PR China. Electronic address: xliu@swjtu.edu.cn.
⁵ Key Laboratory of Superlight Materials & Surface Technology, Ministry of Education, College of Materials Science and Chemical Engineering, Harbin Engineering University, Harbin 150001, PR China; Yantai Research Institute of Harbin Engineering University, Yantai, 264006, PR China.
⁶ State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China. Electronic address: shiqiang@ciac.ac.cn.

PMID: 38151078
DOI: 10.1016/j.ijbiomac.2023.128960

Abstract

Herein, a novel bioinspired radial porous zinc-based metal-organic framework (Zn-MOF) doped sodium alginate/chitosan derivatives/pullulan-based SA/PSCS/Pul/Zn-MOF (SPCP/Zn) composites sponge with excellent antioxidant and antibacterial properties was fabricated by the ice-templating method. Boric acid (BA) and Ca²⁺, which were respectively used as hydrogen- and ionic- bonding cross-linkers, provided strong mechanical properties for sponge matrix composed of SA, PSCS, and Pul. The obtained SPCP/Zn sponge exhibited uniform porous morphology, proper hydrophilicity, and admirable biocompatibility. In addition, the SPCP/Zn sponge achieved a sustained release of Zn²⁺ and gallic acid, which displayed powerful antibacterial and antioxidant activities. Importantly, the SPCP/Zn sponge exhibited shorter rapid hemostasis (20.4 ± 2.9 s) and lower blood loss (19.8 ± 4.3 mg). The SPCP/Zn sponge also showed faster wound closure ratio for the rat full-thickness skin defect model. It was revealed that SPCP/Zn sponge could significantly accelerate and enhance wound healing through downregulating inflammatory cytokines (TNF-α, IL-6) and increasing the expression of growth factors (VEGF). Due to its excellent properties, the SPCP/Zn sponge may have promising potential in wound healing applications.

Keywords: Antioxidant; Bioinspired; Wound healing.

MeSH terms

Animals
Anti-Bacterial Agents / pharmacology
Antioxidants / pharmacology
Chitosan* / pharmacology
Hemostasis
Metal-Organic Frameworks* / pharmacology
Porosity
Rats
Wound Healing
Zinc

Substances

Antioxidants
Chitosan
Metal-Organic Frameworks
Zinc
Anti-Bacterial Agents