Regulatory mechanism of the six-method massage antipyretic process on lipopolysaccharide-induced fever in juvenile rabbits: A targeted metabolomics approach

Di Liu; Ying-Qi Zhang; Tian-Yuan Yu; Si-Long Han; Ya-Jing Xu; Qian Guan; Hou-Rong Wang

doi:10.1016/j.heliyon.2023.e23313

Regulatory mechanism of the six-method massage antipyretic process on lipopolysaccharide-induced fever in juvenile rabbits: A targeted metabolomics approach

Heliyon. 2023 Dec 8;10(1):e23313. doi: 10.1016/j.heliyon.2023.e23313. eCollection 2024 Jan 15.

Authors

Di Liu^{1

2}, Ying-Qi Zhang¹, Tian-Yuan Yu¹, Si-Long Han³, Ya-Jing Xu¹, Qian Guan¹, Hou-Rong Wang¹

Affiliations

¹ School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, 102401, China.
² Department of Acupuncture-Moxibustion, Dongfang Hospital Affiliated to Beijing University of Chinese Medicine, Beijing, 100078, China.
³ Department of Orthopedics II, Changping District Hospital of Integrated Traditional Chinese and Western Medicine, Beijing,102208, China.

Abstract

Objective: To investigate the mechanism of the six-method massage antipyretic process (SMAP) and its influence on the body's metabolic state.

Methods: The random number table method was used to divide 24 New Zealand 2-month-old rabbits with qualified basal body temperature into a control group, model group and massage group (n = 8 per group). The model group and massage groups were injected with 0.5 μg/ml lipopolysaccharide (1 ml/kg) into the auricular vein, and the control group was injected with the same amount of normal saline at the same temperature. One hour after modelling, the massage group was given SMAP (opening Tianmen, pushing Kangong, rubbing Taiyang, rubbing Erhougaogu, clearing the Tianheshui and pushing the spine). The change of anal temperature 5 h after moulding was recorded to clarify the antipyretic effect.

Results: After modelling, the rectal temperature of the juvenile rabbits in the three groups increased. The rectal temperature of the model group was higher than that of the control group 5 h after modelling, and the rectal temperature of the massage group was lower than that of the model group (P < 0.05). The antipyretic mechanism is related to the regulation of the synthesis of phenylalanine, tyrosine and tryptophan, as well as the pentose phosphate pathway. Compared with the model group, the plasma interleukin (IL)-1, IL-6, interferon-gamma, toll-like receptor 4, nuclear factor κB, the mechanistic target of rapamycin complex 1, indoleamine 2,3-dioxygenase 1, aryl hydrocarbon receptor, liver aspartate transaminase (AST), alanine transaminase (ALT) and l-glutamate dehydrogenase (L-GLDH) expression in the massage group were significantly decreased (P < 0.05). Compared with the model group, the massage group had significantly reduced AST, ALT and L-GLDH expression in plasma (P < 0.05).

Conclusion: The mechanism of SMAP therapy is related to regulating the expression of peripheral inflammatory factors and metabolic pathways.

Keywords: Amino acid metabolism; Pentose phosphate pathway; infantile massage; metabolomics; tryptophan.