Randomly barcoded transposon mutant libraries for gut commensals II: Applying libraries for functional genetics

Cell Rep. 2024 Jan 23;43(1):113519. doi: 10.1016/j.celrep.2023.113519. Epub 2023 Dec 23.

Abstract

The critical role of the intestinal microbiota in human health and disease is well recognized. Nevertheless, there are still large gaps in our understanding of the functions and mechanisms encoded in the genomes of most members of the gut microbiota. Genome-scale libraries of transposon mutants are a powerful tool to help us address this gap. Recent advances in barcoded transposon mutagenesis have dramatically lowered the cost of mutant fitness determination in hundreds of in vitro and in vivo experimental conditions. In an accompanying review, we discuss recent advances and caveats for the construction of pooled and arrayed barcoded transposon mutant libraries in human gut commensals. In this review, we discuss how these libraries can be used across a wide range of applications, the technical aspects involved, and expectations for such screens.

Keywords: CP: Microbiology; chemical genomics; genotype-phenotype mapping; gut microbiota; host-microbe interactions; transposon mutagenesis.

Publication types

  • Review
  • Research Support, Non-U.S. Gov't
  • Research Support, N.I.H., Extramural

MeSH terms

  • DNA Transposable Elements* / genetics
  • Gene Library
  • Humans
  • Mutagenesis, Insertional / genetics

Substances

  • DNA Transposable Elements