Soybean 40S Ribosomal Protein S8 (GmRPS8) Interacts with 6K1 Protein and Contributes to Soybean Susceptibility to Soybean Mosaic Virus

Ting Hu; Dongquan Guo; Bowen Li; Liqun Wang; Hui Liu; Jinlong Yin; Tongtong Jin; Hexiang Luan; Lei Sun; Mengzhuo Liu; Haijian Zhi; Kai Li

doi:10.3390/v15122362

Soybean 40S Ribosomal Protein S8 (GmRPS8) Interacts with 6K1 Protein and Contributes to Soybean Susceptibility to Soybean Mosaic Virus

Viruses. 2023 Nov 30;15(12):2362. doi: 10.3390/v15122362.

Authors

Ting Hu¹, Dongquan Guo², Bowen Li¹, Liqun Wang¹, Hui Liu¹, Jinlong Yin¹, Tongtong Jin¹, Hexiang Luan³, Lei Sun¹, Mengzhuo Liu¹, Haijian Zhi¹, Kai Li¹

Affiliations

¹ Key Laboratory of Biology and Genetics Improvement of Soybean, Ministry of Agriculture/Zhongshan Biological Breeding Laboratory (ZSBBL)/National Innovation Platform for Soybean Breeding and Industry-Education Integration/State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization/College of Agriculture, Nanjing Agricultural University, Nanjing 210095, China.
² Jilin Academy of Agricultural Sciences, Changchun 130033, China.
³ Institute of Plant Genetic Engineering, College of Life Science, Qingdao Agricultural University, Qingdao 266109, China.

Abstract

Soybean mosaic virus (SMV), a member of Potyvirus, is the most destructive and widespread viral disease in soybean production. Our earlier studies identified a soybean 40S ribosomal protein S8 (GmRPS8) using the 6K1 protein of SMV as the bait to screen a soybean cDNA library. The present study aims to identify the interactions between GmRPS8 and SMV and characterize the role of GmRPS8 in SMV infection in soybean. Expression analysis showed higher SMV-induced GmRPS8 expression levels in a susceptible soybean cultivar when compared with a resistant cultivar, suggesting that GmRPS8 was involved in the response to SMV in soybean. Subcellular localization showed that GmRPS8 was localized in the nucleus. Moreover, the yeast two-hybrid (Y2H) experiments showed that GmRPS8 only interacted with 6K1 among the eleven proteins encoded by SMV. The interaction between GmRPS8 and 6K1 was further verified by a bimolecular fluorescence complementation (BiFC) assay, and the interaction was localized in the nucleus. Furthermore, knockdown of GmRPS8 by a virus-induced gene silencing (VIGS) system retarded the growth and development of soybeans and inhibited the accumulation of SMV in soybeans. Together, these results showed that GmRPS8 interacts with 6K1 and contributes to soybean susceptibility to SMV. Our findings provide new insights for understanding the role of GmRPS8 in the SMV infection cycle, which could help reveal potyviral replication mechanisms.

Keywords: disease susceptibility; growth; protein interaction; virus-induced gene silencing.

MeSH terms

Glycine max* / genetics
Plant Diseases
Potyvirus* / genetics

Substances

ribosomal protein S8

Supplementary concepts

Soybean mosaic virus

Grants and funding

This research was funded by grants from the analysis on the genetic mechanism of the resistance of the major food crops to pests and diseases (the National Key Research and Development Program of China 2022YFF1001500), the Core Technology Development for Breeding Program of Jiangsu Province (JBGS-2021-014), the China Agriculture Research System of MOF and MARA (No. CARS-04), the Jiangsu Collaborative Innovation Center for Modern Crop Production (JCIC-MCP), the Collaborative Innovation Center for Modern Crop Production co-sponsored by the Province and Ministry (CIC-MCP), and the Fundamental Research Funds for the Central Universities.