Zuccagnia punctata Cav., a Potential Environmentally Friendly and Sustainable Bionematicide for the Control of Argentinean Horticultural Crops

Sofía Manrique; Jessica Gómez; Mauricio Piñeiro; Belén Ariza Sampietro; Maria L Peschiutta; Alejandro Tapia; Mario J Simirgiotis; Beatriz Lima

doi:10.3390/plants12244104

Zuccagnia punctata Cav., a Potential Environmentally Friendly and Sustainable Bionematicide for the Control of Argentinean Horticultural Crops

Plants (Basel). 2023 Dec 7;12(24):4104. doi: 10.3390/plants12244104.

Authors

Sofía Manrique^{1

2}, Jessica Gómez^{1

2}, Mauricio Piñeiro^{1

2}, Belén Ariza Sampietro^{1

2}, Maria L Peschiutta³, Alejandro Tapia¹, Mario J Simirgiotis^{4

5}, Beatriz Lima^{1

2}

Affiliations

¹ Instituto de Biotecnología-Instituto de Ciencias Básicas, Universidad Nacional de San Juan (UNSJ), San Juan J5400ARL, Argentina.
² Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), CABA, Buenos Aires C1425FQB, Argentina.
³ Instituto Multidisciplinario de Biología Vegetal (IMBIV-CONICET)-Cátedra de Química Orgánica, Facultad de Ciencias Exactas, Físicas y Naturales, Universidad Nacional de Córdoba, Córdoba X5000GYA, Argentina.
⁴ Instituto de Farmacia, Facultad de Ciencias, Campus Isla Teja, Universidad Austral de Chile, Valdivia 5090000, Chile.
⁵ Center for Interdisciplinary Studies on the Nervous System (CISNe), Universidad Austral de Chile, Valdivia 5090000, Chile.

Abstract

This research was designed to investigate the metabolite profiling, phenolics, and flavonoids content as well as the potential nematicidal properties of decoction (ZpDe), orange-yellow resin (ZpRe) and essential oil (ZpEO) from Argentinean medicinal plant Zuccagnia punctata Cav. Additionally, the antioxidant and antibacterial properties of ZpDe and ZpEO were determined. Metabolite profiling was obtained by an ultrahigh-resolution liquid chromatography MS analysis (UHPLC-ESI-QTOF/OT-MS-MS) and GCMS. The nematicidal activity was assayed by a standardized method against Meloidogyne incognita. The antioxidant properties were screened by four methods: (2,2-diphenyl-1-picrylhydrazyl assay (DPPH), Trolox equivalent antioxidant activity assay (TEAC), ferric-reducing antioxidant power assay (FRAP), and lipid peroxidation in erythrocytes (ILP). The antibacterial activity was evaluated according to the Clinical and Laboratory Standards Institute (CLSI) rules. The ZpDe, ZpRe and ZpEO displayed a strong nematicidal activity with an LC₅₀ of 0.208, 0.017 and 0.142 mg/mL, respectively. On the other hand, the ZpDe showed a strong DPPH scavenging activity (IC₅₀ = 28.54 µg/mL); ILP of 87.75% at 250 µg ZpDe/mL and moderated antimicrobial activity. The ZpEO showed promising activity against a panel of yeasts Candida albicans and non-albicans (ATCC and clinically isolated) with MIC values from 750 to 1500 µg/mL. The ZpDe showed a content of phenolics and flavonoid compounds of 241 mg GAE/g and 10 mg EQ/g, respectively. Fifty phenolic compounds were identified in ZpDe by ultrahigh-resolution liquid chromatography (UHPLC-PDA- Q-TOF-MS) analysis, while forty-six phenolic compounds were identified in ZpRe by UHPLC-ESI-Q-OT-MS-MS and twenty-nine in ZpEO using a GC-MS analysis, updating the knowledge on the chemical profile of this species. The results support and standardize this medicinal plant mainly as a potential environmentally friendly and sustainable bionematicide for the control of Argentinean horticultural crops including tomatoes and peppers and as a source of antimicrobial and antioxidant compounds which could be further explored and exploited for potential applications.

Keywords: Meloidogyne incognita; antioxidant; decoction; essential oil; resin exudate.

Grants and funding

This research was funded by PROJOVI-UNSJ (Nº 1500-23-R)- and PDTS-UNSJ (I/1613); Argentina. M.J.S. received financial support from Fondecyt (Grant 1220075) and fondequip (EQM140002), Chile.