Detection of Porcine Circovirus Type 3 in Serum, Semen, Oral Fluid, and Preputial Fluid Samples of Boars

Song Qi; Qiyun He; Zhewei Zhang; Huanchun Chen; Luis Giménez-Lirola; Fangyan Yuan; Weicheng Bei

doi:10.3390/vetsci10120689

Detection of Porcine Circovirus Type 3 in Serum, Semen, Oral Fluid, and Preputial Fluid Samples of Boars

Vet Sci. 2023 Dec 4;10(12):689. doi: 10.3390/vetsci10120689.

Authors

Song Qi^{1

2}, Qiyun He^{1

2}, Zhewei Zhang^{1

2}, Huanchun Chen^{1

2

3}, Luis Giménez-Lirola⁴, Fangyan Yuan⁵, Weicheng Bei^{1

2

3}

Affiliations

¹ National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430000, China.
² The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430000, China.
³ Hubei Hongshan Laboratory, Wuhan 430000, China.
⁴ Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA.
⁵ Hubei Key Laboratory of Animal Embryo and Molecular Breeding, Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan 430000, China.

Abstract

Porcine circovirus type 3 (PCV3) is commonly associated with clinical symptoms such as porcine dermatitis and nephropathy syndrome (PDNS)-like lesions, respiratory signs, and reproductive disorders. This study aimed to investigate the epidemiology of PCV3 in a boar stud. The objectives were to detect PCV3 in semen, as well as matched serum, oral fluid, and preputial fluid samples from adult boars using quantitative polymerase chain reaction (qPCR), analyze PCV3-IgG antibody data, and genetically characterize a positive sample. A total of 112 samples from 28 boars were collected from a large-scale pig farm in Guangxi, China. The qPCR results showed that the PCV3 DNA was not detected in semen, with a positive rate of 0% (0/28), while it was detected in serum (3.57%-1/28), oral fluid (64.28%-18/28), and preputial fluid (46.4%-13/28). The seropositivity rate of PCV3-IgG in serum was 82.14% (23/28) according to the indirect enzyme-linked immunosorbent serologic assay (ELISA) results. Phylogenetic analysis revealed that one of the PCV3 isolates belonged to the PCV3c clades. This is the first report of PCV3 detection in preputial fluid from boars. The results suggest that PCV3 is transmitted among boars on pig farms and exhibits epidemic characteristics.

Keywords: PCV3; oral fluid; preputial fluid; semen; serum.

Grants and funding

This work was supported by grants from the National Key Research and Development Program of China (2021YFD1800400), the National Key Research and Development Program of China (2022YFD1800905), the National Natural Science Foundation of China (32273039).