Assessment of measurement accuracy of amplified DNA using a colorimetric loop-mediated isothermal amplification assay

Biotechniques. 2024 Mar;76(3):114-118. doi: 10.2144/btn-2023-0104. Epub 2023 Dec 22.

Abstract

A colorimetric loop-mediated isothermal amplification assay detects changes in pH during amplification based on color changes at a constant temperature. Currently, various studies have focused on developing and assessing molecular point-of-care testing instruments. In this study, we evaluated amplified DNA concentrations measured using the colorimetric LAMP assay of the 1POT™ Professional device (1drop Inc, Korea). Results of the 1POT analysis of clinical samples were compared with measurements obtained from the Qubit™ 4 and NanoDrop™ 2000 devices (both from Thermo Fisher Scientific, MA, USA). These results showed a correlation of 0.98 (95% CI: 0.96-0.99) and 0.96 (95% CI: 0.92-0.98) between 1POT and the Qubit and NanoDrop. 1POT can measure amplified DNA accurately and is suitable for on-site molecular diagnostics.

Keywords: 1POT™; NanoDrop™; Qubit™; loop-mediated isothermal amplification; point-of-care testing device.

MeSH terms

  • Colorimetry*
  • DNA* / analysis
  • DNA* / genetics
  • Molecular Diagnostic Techniques / methods
  • Nucleic Acid Amplification Techniques / methods
  • Sensitivity and Specificity

Substances

  • DNA

Supplementary concepts

  • LAMP assay