Characterization of chloroplast genome and phylogenetic analysis of the Elaeagnus hybrid variety E. 'viridis' (Elaeagnaceae)

Naiwei Li; Hong Chen; Yanwei Zhou; Xinran Chong; Ting Zhou; Fan Zhang

doi:10.1080/23802359.2023.2292156

Characterization of chloroplast genome and phylogenetic analysis of the Elaeagnus hybrid variety E. 'viridis' (Elaeagnaceae)

Mitochondrial DNA B Resour. 2023 Dec 18;8(12):1416-1420. doi: 10.1080/23802359.2023.2292156. eCollection 2023.

Authors

Naiwei Li¹, Hong Chen^{1

2}, Yanwei Zhou^{1

2}, Xinran Chong^{1

2}, Ting Zhou^{1

2}, Fan Zhang^{1

2}

Affiliations

¹ Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, China.
² Jiangsu Key Laboratory for the Research and Utilization of Plant Resources, Nanjing, China.

Abstract

Elaeagnus 'viridis', an artificial hybrid of E. macrophylla (♂) Thunb. (1784) × E. pungens (♀) Thunb. (1784), is known for its economic and ecological value. In this study, we sequenced and assembled the whole chloroplast (cp) genome of E. 'viridis'. The results showed that its cp genome was 152,284 bp long, showing a typical quadripartite structure and containing a large single-copy region (LSC, 82,299 bp), a small single-copy region (SSC, 18,239 bp), and a pair of inverted repeats (IRs, 51,746 bp). The cp genome contains 132 genes, including 86 protein-coding genes (PCGs), 38 tRNA genes, and 8 rRNA genes. Phylogenetic analysis based on 66 common PCGs revealed that E. 'viridis' is most closely related to its maternal parent E. pungens. The chloroplast genomic information reported in this study will shed some useful light for further genetic studies in the genus Elaeagnus.

Keywords: Elaeagnus ‘viridis’; complete chloroplast genome; phylogenetic analysis.

Grants and funding

This work was supported by the Public Science and Technology Research Funds Project of Oceans [grant number: 201505023], the Natural Science Foundation of Jiangsu Province [grant no. BK20201244], and the Natural Science Foundation of Jiangsu Province [grant no. BK20210163]