Background: Hypochlorite/hypochlorous acid (ClO-/HOCl) is a biologically crucial reactive oxygen species (ROS), produced in living organisms and has a critical role as an antimicrobial agent in the natural defense system. However, when ClO- is produced excessively, it can lead to the oxidative damage of biomolecules, resulting in organ damage and various diseases. Therefore, it is imperative to have a straightforward, quick and reliable method for over watching the minimum amount of ClO- in different environments.
Results: Herein, a new probe TTM, containing thienothiophene and malononitrile units, was developed for exceptionally selective and sensitive hypochlorite (ClO-) detection. TTM demonstrated a rapid "turn-on" fluorescence response (<30 s), naked-eye detection (colorimetric), voltammetric read-out with anodic scan, low detection limit (LOD = 0.58 μM and 1.43 μM for optical and electrochemical methods, respectively) and applicability in detecting ClO- in real water samples and living cells.
Significance and novelty: This study represents one of the rare examples of a small thienothiophene-based molecule for both optical and electrochemical detections of ClO- in an aqueous medium.
Keywords: Cell imaging; Cyclic voltammetry; Hypochlorite detection; Malononitrile; Thienothiophene.
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