Two new polythioamides were prepared through the polycondensation reaction between thiourea monomers and terephthaloyl dichloride, while the thiourea monomers were synthesized by the interaction of aromatic (4,4'-diaminophenylsulfone) or alicyclic (1,2-cyclohexanediamine) diamine with ammonium thiocyanate. The elemental composition of polythioamides was confirmed through CHN microanalysis. The structure and properties of thiourea monomers and polythioamides were determined through proton NMR, UV-Vis, FT-IR spectroscopy, fluorescence, TGA/DTA and SEM. The polythioamides indicated high thermal stabilities which were assessed from their Tmax (temperature indicating highest rate of weight loss) values (670 °C and 346 °C) observed in their DTG graphs. The thioureas and polythioamides were fluorescent and showed multicolor (violet, green, yellow, orange and red) emissions at different excitation wavelengths. All the synthesized compounds were also tested for their antifungal and antibacterial functions and showed antibacterial activity against Salmonella typhi, Bacillus subtilis and Staphylococcus aureus, and antifungal activity against Candida albicans.