Synaptic plasticity via receptor tyrosine kinase/G-protein-coupled receptor crosstalk

Cell Rep. 2024 Jan 23;43(1):113595. doi: 10.1016/j.celrep.2023.113595. Epub 2023 Dec 19.

Abstract

Cellular signaling involves a large repertoire of membrane receptors operating in overlapping spatiotemporal regimes and targeting many common intracellular effectors. However, both the molecular mechanisms and the physiological roles of crosstalk between receptors, especially those from different superfamilies, are poorly understood. We find that the receptor tyrosine kinase (RTK) TrkB and the G-protein-coupled receptor (GPCR) metabotropic glutamate receptor 5 (mGluR5) together mediate hippocampal synaptic plasticity in response to brain-derived neurotrophic factor (BDNF). Activated TrkB enhances constitutive mGluR5 activity to initiate a mode switch that drives BDNF-dependent sustained, oscillatory Ca2+ signaling and enhanced MAP kinase activation. This crosstalk is mediated, in part, by synergy between Gβγ, released by TrkB, and Gαq-GTP, released by mGluR5, to enable physiologically relevant RTK/GPCR crosstalk.

Keywords: BDNF; CP: Cell biology; CP: Neuroscience; G-protein-coupled receptor; GBA motif; TrkB; calcium signaling; metabotropic glutamate receptor; neuromodulation; neurotrophin; receptor tyrosine kinase; synaptic plasticity.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Brain-Derived Neurotrophic Factor*
  • Neuronal Plasticity / physiology
  • Receptor Protein-Tyrosine Kinases*
  • Receptor, trkB / metabolism
  • Receptors, G-Protein-Coupled
  • Signal Transduction / physiology

Substances

  • Brain-Derived Neurotrophic Factor
  • Receptor Protein-Tyrosine Kinases
  • Receptor, trkB
  • Receptors, G-Protein-Coupled