<b>Background and Objective:</b> Probiotic yogurt is beneficial for laying hens because it can improve the animal's hematological status which will improve livestock health, therefore it is hoped that probiotic yogurt can increase the production of laying hens. This research was conducted to determine the lactic acid levels and pH of probiotic yogurt, probiotic yogurt's effect on feed conversion ratio and total production of laying hens. <b>Materials and Methods:</b> The research was carried out using experimental methods using a Completely Randomized Design (CRD) with 5 treatments and 8 replications so the total sample was 40. The treatment consisted of P0: Basal ration; P1: Basal diet+2% probiotic powder B1 (<i>Bifidobacterium</i> spp. and <i>L. acidophilus</i>), P2: Basal ration+3% probiotic powder B1, P3: Basal diet+2% probiotic powder B2 (<i>L. bulgaricus</i>, <i>S. thermophilus</i>, <i>L. acidophilus</i> and <i>B. bifidum</i>) and P4: Basal ration+3% probiotic powder B2. The data were analyzed using Analysis of Variance (ANOVA) and followed by Duncan's Multiple Range Test. <b>Results:</b> Lactic acid content in probiotic yogurts B1 is 0.945% and B2 is 0.638%. Based on the results of statistical analysis using the variance test, show that giving probiotic powder to laying hens has a significant effect on the feed conversion ratio and has no significant effect on the production of laying hens. <b>Conclusion:</b> Based on the results of statistical analysis using the variance test, it shows that giving probiotic powder to laying hens has no significant effect on the production of laying hens during the peak period.
Keywords: Laying hens; egg production; feed conversion ratio; lactic acid; pH; probiotic powder.