Simultaneous remediation of diesel-polluted soil and promoted ryegrass growth by non-thermal plasma pretreatment

Abstract

The remediation of petroleum-polluted soil has garnered significant global attention. In this study, a pot-culture experiment was conducted to assess the feasibility of using non-thermal plasma (NTP) as an efficient and economic-friendly pretreatment method in the phytoremediation of diesel-polluted soil. The remediation effectiveness was evaluated via both the removal of diesel and the ryegrass growth. Specifically, at the 50th d of ryegrass growth, the increase of diesel removal efficiency with NTP pretreatment ranged from 16 % to 30 %. Moreover, both clean and diesel-polluted soils pretreated by NTP promoted the growth of ryegrass in shoot lengths and biomass especially after the 35th d. It was found that nitrate nitrogen fixed by NTP not only stimulated the nitrate reductase activities in leaves and promoted plant growth, but also was transformed to more ammonia nitrogen for organism life activity. Subsequent investigation proved that the related nitrogen-metabolism activities of microbes were enriched in rhizosphere soils with NTP pretreatment. Furthermore, NTP treatment increased the abundance of beneficial microbial communities in diesel soil rhizosphere on the 42nd d of growth period. In addition, changes in the proportions of soil dissolved organic matter indicated enhanced nutrient cycling in soils with NTP pretreatment. These promotional effects underscored the contribution of NTP pretreatment in rapidly detoxifying diesel-contaminated soil within 10 min and accelerated the establishment of ryegrass ecosystem. This study provides valuable insights into the role of nitrogen fixation and offers an efficient and promising advanced approach for the phytoremediation of diesel-polluted soil with NTP pretreatment.

Keywords: Dissolved organic matter; Inorganic nitrogen; Nitrate reductase activities; Phytoremediation; Rhizosphere microbes.