Anti-allergic effects of Ulva-derived polysaccharides, oligosaccharides and residues in a murine model of food allergy

Jing-Yi Ou; Yu-Jyun Wei; Fang-Ling Liu; Chung-Hsiung Huang

doi:10.1016/j.heliyon.2023.e22840

Anti-allergic effects of Ulva-derived polysaccharides, oligosaccharides and residues in a murine model of food allergy

Heliyon. 2023 Nov 26;9(12):e22840. doi: 10.1016/j.heliyon.2023.e22840. eCollection 2023 Dec.

Authors

Jing-Yi Ou¹, Yu-Jyun Wei¹, Fang-Ling Liu¹, Chung-Hsiung Huang^{1

2}

Affiliations

¹ Department of Food Science, National Taiwan Ocean University, Keelung 20224, Taiwan.
² Center for Marine Bioscience and Biotechnology, National Taiwan Ocean University, Keelung 20224, Taiwan.

Abstract

The medicinal benefits of green seaweed Ulva have been documented in traditional Chinese medicine literatures. Sulfated polysaccharides found in Ulva are recognized as the primary bioactive compounds, known for their immunomodulatory and anti-inflammatory properties. Despite this knowledge, the available information regarding anti-allergic activities of Ulva remains limited. The objective of this study was to prepare and characterize Ulva-derived polysaccharides (UP), oligosaccharides (UO), and residues (UR), followed by assessing their potential in improving allergic enteritis and gut microbiota in a murine model of ovalbumin (OVA)-induced food allergy. The immunomodulatory activities of UP, UO, and UR were evaluated by measuring the expression of serum antibodies, splenic cytokines and duodenal transcript factors of T cell subsets. The impact of UP, UO, and UR on enteric microbiota was explored by 16S rRNA gene sequencing analysis of fresh fecal samples from treated mice. Oral treatment of UP, UO, and UR noticeably attenuated allergic diarrhea and enteritis. Additionally, Ulva samples treatment decreased serum levels of IgG₁ and OVA-specific IgE while increased the level of OVA-specific IgG. Enhanced production of IFN-γ and reduced production of IL-4 and IL-10 by splenocytes were observed in the treated mice. In parallel, Ulva samples treatment led to a decreased number of GATA3⁺ cells and an increased number of T-bet⁺ cells in the duodenum. However, the population of Foxp3⁺ cells was not significantly altered. Moreover, treatment of Ulva samples improved enteric dysbiosis evidenced by an increased abundance of Lactobacillus murinus, L. johnsonii, and L. reuteri, and a decreased abundance of Kineothrix alysoides, Lacrimispora saccharolytica, L. aerotolerans, and Erysipelotrichaceae in feces. In conclusion, UP, UO, and UR, which could modulate the Th1/Th2 immune balance, alleviate allergic enteritis and improve enteric dysbiosis in varying degrees, are potential to be developed into therapeutic agents for food allergy.

Keywords: Dysbiosis; Food allergy; Oligosaccharides; Polysaccharides; Residues; Ulva.