Enlarging the scenario of site directed 19F labeling for NMR spectroscopy of biomolecules

Abstract

The possibility of using selectively incorporated ¹⁹F nuclei for NMR spectroscopic studies has retrieved increasing interest in recent years. The high gyromagnetic ratio of ¹⁹F and its absence in native biomolecular systems make this nucleus an interesting alternative to standard ¹H NMR spectroscopy. Here we show how we can attach a label, carrying a ¹⁹F atom, to protein tyrosines, through the use of a specific three component Mannich-type reaction. To validate the efficacy and the specificity of the approach, we tested it on two selected systems with the aid of ESI MS measurements.