The Interaction of SRL-2 Peptide with LRP-1 Receptor and Identification of Breast Cancer Related Biomarkers: An In-silico Approach

Yasamin Davatgaran-Taghipour; Yousof Saeedi-Honar; Roya Salehi; Amir Zarebkohan; Vladimir P Torchilin

doi:10.5812/ijpr-136624

The Interaction of SRL-2 Peptide with LRP-1 Receptor and Identification of Breast Cancer Related Biomarkers: An In-silico Approach

Iran J Pharm Res. 2023 Jun 11;22(1):e136624. doi: 10.5812/ijpr-136624. eCollection 2023 Jan-Dec.

Authors

Yasamin Davatgaran-Taghipour^{1

2}, Yousof Saeedi-Honar³, Roya Salehi^{2

4}, Amir Zarebkohan^{2

4}, Vladimir P Torchilin⁵

Affiliations

¹ Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.
² Department of Medical Nanotechnology, School of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
³ Department of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran.
⁴ Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
⁵ Center for Pharmaceutical Biotechnology and Nanomedicine and Department of Chemical Engineering, Northeastern University, Boston, USA.

Abstract

Background: Breast cancer is a multifaceted disease characterized by genetic and epigenetic changes that lead to uncontrolled cell growth and metastasis. Early detection and treatment are crucial for managing diseases.

Objectives: The objective of this study is to investigate the potential of chimeric peptides for drug delivery and to identify biomarkers associated with breast cancer. Recent studies have shown that the low-density lipoprotein receptor-related protein 1 (LRP-1) receptor has a significant impact on the development of breast cancer. In order to facilitate the identification of biomarkers, we have created a chimeric peptide that has been proven to bind successfully to the LRP-1 receptor.

Methods: To identify biomarkers, we utilized advanced computational methods to conduct a meta-analysis of microarray data. Specifically, the g:Profiler and eXpression2Kinases (X2K) databases were utilized to identify gene ontologies and transcription factors. We then used the Human Protein Atlas to identify and assess crucial gene expressions.

Results: Our results demonstrated that nucleolar and spindle-associated protein 1 (NUSAP1), melatonin receptor 1A (MELT), and cyclin-dependent kinase 1 (CDK1) are three hub genes that play pivotal roles in the pathogenesis of breast cancer.

Conclusions: The research findings provide a deeper understanding of the molecular mechanisms involved in developing breast cancer. These findings have significant implications for developing novel therapies and diagnostics for this disease.

Keywords: Biomarkers; Breast Cancer; Chimeric Peptide; LRP-1; Meta-analysis.