Structure of Transmembrane AMPA Receptor Regulatory Protein Subunit γ2

W Dylan Hale; Alejandra Montaño Romero; Richard L Huganir; Edward C Twomey

doi:10.1101/2023.11.28.569079

Structure of Transmembrane AMPA Receptor Regulatory Protein Subunit γ2

bioRxiv [Preprint]. 2023 Nov 28:2023.11.28.569079. doi: 10.1101/2023.11.28.569079.

Authors

W Dylan Hale^{1

2}, Alejandra Montaño Romero^{1

2}, Richard L Huganir^{1

3}, Edward C Twomey^{1

2

4

5}

Affiliations

¹ Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD USA.
² Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD USA.
³ Kavli Neuroscience Discovery Institute, Johns Hopkins University School of Medicine, Baltimore, MD USA.
⁴ The Beckman Center for Cryo-EM at Johns Hopkins, Johns Hopkins University School of Medicine, Baltimore, MD USA.
⁵ Diana Helis Henry Medical Research Foundation, New Orleans, LA USA.

Abstract

Transmembrane AMPA receptor regulatory proteins (TARPs) are claudin-like proteins that tightly regulate AMPA receptors (AMPARs) and are fundamental for excitatory neurotransmission. We used cryo-electron microscopy (cryo-EM) to reconstruct the 36 kDa TARP subunit γ2 to 2.3 Å and reveal the structural diversity of TARPs. Our data reveals critical motifs that distinguish TARPs from claudins and define how sequence variations within TARPs differentiate subfamilies and their regulation of AMPARs.

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Preprint

Abstract

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