Retroviral Transduction of Human Primary T Cells Followed by Real-Time T-Cell-Mediated Cancer Cell Cytolysis Analysis

Anne Rahbech; Reno Debets; Per Thor Straten; Marlies J W Peeters

doi:10.1007/978-1-0716-3593-3_3

Retroviral Transduction of Human Primary T Cells Followed by Real-Time T-Cell-Mediated Cancer Cell Cytolysis Analysis

Methods Mol Biol. 2024:2748:29-39. doi: 10.1007/978-1-0716-3593-3_3.

Authors

Anne Rahbech¹, Reno Debets², Per Thor Straten^{1

3}, Marlies J W Peeters⁴

Affiliations

¹ Department of Oncology, National Center for Cancer Immune Therapy, University Hospital Herlev, Copenhagen, Denmark.
² Laboratory of Tumor Immunology, Department of Medical Oncology, Erasmus MC-Cancer Center, Rotterdam, The Netherlands.
³ Department of Immunology and Microbiology, Inflammation and Cancer Group, University of Copenhagen, Copenhagen, Denmark.
⁴ Department of Oncology, National Center for Cancer Immune Therapy, University Hospital Herlev, Copenhagen, Denmark. marlies.peeters@regionh.dk.

PMID: 38070105
DOI: 10.1007/978-1-0716-3593-3_3

Abstract

Retroviral transduction is a highly useful tool to genetically engineer hard-to-transfect human primary cells. Here, we transduce human primary T cells with a tumor-specific T cell receptor. This creates a useful tool to analyze T cell-cancer cell interactions, such as cytolysis analysis using xCELLigence technology.

Keywords: Cytolysis; Retrovirus; T cells; Transduction; xCELLigence.

MeSH terms

Genetic Vectors
Humans
Neoplasms*
Receptors, Antigen, T-Cell / genetics
Retroviridae / genetics
T-Lymphocytes*
Transduction, Genetic

Substances

Receptors, Antigen, T-Cell