Cucumis sativus CsbZIP90 suppresses Podosphaera xanthii resistance by modulating reactive oxygen species

Linghao Liu; Lifeng Ma; Yongbo Yu; Zhangtong Ma; Yunhan Yin; Shuang Zhou; Yang Yu; Na Cui; Xiangnan Meng; Haiyan Fan

doi:10.1016/j.plantsci.2023.111945

Cucumis sativus CsbZIP90 suppresses Podosphaera xanthii resistance by modulating reactive oxygen species

Plant Sci. 2024 Feb:339:111945. doi: 10.1016/j.plantsci.2023.111945. Epub 2023 Dec 6.

Authors

Linghao Liu¹, Lifeng Ma¹, Yongbo Yu¹, Zhangtong Ma¹, Yunhan Yin¹, Shuang Zhou¹, Yang Yu², Na Cui², Xiangnan Meng³, Haiyan Fan⁴

Affiliations

¹ College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866, China.
² College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866, China; Key Laboratory of Fruit and Vegetable Biology and Germplasm Enhancement, Shenyang Agricultural University, Shenyang 110866, China; Key Laboratory of Protected Horticulture of Ministry of Education, Shenyang Agricultural University, Shenyang 110866, China.
³ College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866, China; Key Laboratory of Fruit and Vegetable Biology and Germplasm Enhancement, Shenyang Agricultural University, Shenyang 110866, China; Key Laboratory of Protected Horticulture of Ministry of Education, Shenyang Agricultural University, Shenyang 110866, China. Electronic address: mengxn@syau.edu.cn.
⁴ College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866, China; Key Laboratory of Fruit and Vegetable Biology and Germplasm Enhancement, Shenyang Agricultural University, Shenyang 110866, China; Key Laboratory of Protected Horticulture of Ministry of Education, Shenyang Agricultural University, Shenyang 110866, China. Electronic address: hyfan74@syau.edu.cn.

PMID: 38061503
DOI: 10.1016/j.plantsci.2023.111945

Abstract

Resistance to disease in plants requires the coordinated action of multiple functionally related genes, as it is difficult to improve disease resistance with a single functional gene. Therefore, the use of transcription factors to regulate the expression of multiple resistance genes to improve disease resistance has become a recent focus in the field of gene research. The basic leucine zipper (bZIP) transcription factor family plays vital regulatory roles in processes, such as plant growth and development and the stress response. In our previous study, CsbZIP90 (Cucsa.134370) was involved in the defense response of cucumber to Podosphaera xanthii, but the relationship between cucumber and resistance to powdery mildew remained unclear. Herein, we detected the function of CsbZIP90 in response to P. xanthii. CsbZIP90 was localized to the cytoplasm and nucleus, and its expression was significantly induced during P. xanthii attack. Transient overexpression of CsbZIP90 in cucumber cotyledons resulted in decreased resistance to P. xanthii, while silencing CsbZIP90 increased resistance to P. xanthii. CsbZIP90 negatively regulated the expression of reactive oxygen species (ROS)-related genes and activities of ROS-related kinases. Taken together, our results show that CsbZIP90 suppresses P. xanthi resistance by modulating ROS. This study will provide target genes for breeding cucumbers resistant to P. xanthii.

Keywords: CsbZIP90; Cucumis sativus L.; Podosphaera xanthii; Reactive oxygen species.

MeSH terms

Ascomycota*
Cucumis sativus* / genetics
Disease Resistance / genetics
Plant Breeding
Plant Diseases / genetics
Reactive Oxygen Species

Substances

Reactive Oxygen Species

Supplementary concepts

Podosphaera xanthii