Using two self-developed InDel panels to explore forensic traits and ancestral components in the Hui group

Xi Yuan; Xi Wang; Qiong Lan; Shuanglin Li; Yifeng Lin; Ming Zhao; Hui Xu; Fanzhang Lei; Chunmei Shen; Bofeng Zhu

doi:10.1016/j.ygeno.2023.110756

Using two self-developed InDel panels to explore forensic traits and ancestral components in the Hui group

Genomics. 2024 Jan;116(1):110756. doi: 10.1016/j.ygeno.2023.110756. Epub 2023 Dec 5.

Authors

Xi Yuan¹, Xi Wang¹, Qiong Lan², Shuanglin Li³, Yifeng Lin¹, Ming Zhao¹, Hui Xu¹, Fanzhang Lei¹, Chunmei Shen⁴, Bofeng Zhu⁵

Affiliations

¹ Guangzhou Key Laboratory of Forensic Multi-Omics for Precision Identification, School of Forensic Medicine, Southern Medical University, Guangzhou, Guangdong, China.
² Guangzhou Key Laboratory of Forensic Multi-Omics for Precision Identification, School of Forensic Medicine, Southern Medical University, Guangzhou, Guangdong, China; Microbiome Medicine Center, Department of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
³ Department of Anatomy and Histology, School of Basic Medical Sciences, Shenzhen University, Shenzhen 518060, China.
⁴ Department of Clinical Laboratory, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong, China. Electronic address: cmshen2004@126.com.
⁵ Guangzhou Key Laboratory of Forensic Multi-Omics for Precision Identification, School of Forensic Medicine, Southern Medical University, Guangzhou, Guangdong, China; Microbiome Medicine Center, Department of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, China; Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi'an Jiaotong University, Xi'an, Shaanxi, China. Electronic address: zhubofeng7372@126.com.

PMID: 38061479
DOI: 10.1016/j.ygeno.2023.110756

Abstract

To address the challenges faced by forensic examiners in degraded DNA analysis, we have developed two different panels for various forensic applications, encompassing an AIM-InDel panel for ancestry inference and a Multi-InDel panel for individual identification, respectively. Herein, the efficiencies of these two panels were tested in the Chinese Hui group. By calculating forensic parameters and simulating family relationships, we verified that the Multi-InDel panel could be an effective tool for individual identification, paternity testing, and could assist in kinship identification in the Hui group. For full siblings, the true positive rate of kinship discrimination was 96.553%, when the threshold of log₁₀LR was 1. The cumulative probability of matching as well as cumulative probability of exclusion were 3.8117 × 10^-26 and 0.999999722, respectively. Meanwhile, we found that the AIM-InDel panel was effective for bio-geographic ancestry inference, with the vast majority of loci contributing significantly to distinguish East Asian, African, and European populations. By studying the population structure of the Hui ethnic minority, the genetic distance to the Beijing Han population was the closest among the 26 reference populations, which was similar to previous reports. In summary, we have developed two panels which can be effectively applied to the Hui group for individual identification, parentage testing and bio-geographic ancestry inference.

Keywords: AIM-InDel; Forensic genetics; Hui group; Multi-InDel; Population genetics.

MeSH terms

China
East Asian People* / genetics
Ethnicity* / genetics
Gene Frequency
Genetics, Population
Humans
INDEL Mutation
Minority Groups*
Phenotype