Quantitative assessment of upper limb motor function aids therapists in providing appropriate rehabilitation strategies, which plays an essential role in post-stroke rehabilitation. Traditional assessments, relying on clinical scales or kinematic metrics, often involve subjective scores or are influenced by compensatory strategies. Recently, the use of muscle synergies, representing simplified neuromuscular control, has emerged as a promising approach for post-stroke assessment. In general, muscle synergies are decomposed into two components: synergy vectors and synergy activation. Synergy vectors represent the relative weighting of each muscle within each synergy, that is muscle coordination; synergy activation represents the recruitment of the muscle synergy over time, that is muscle activation strength. Both components are vital for adequately assessing patients' motor function. Therefore, we integrate the spatial domain and temporal domain features extracted from synergy vectors and synergy activation, constructing a multi-domain assessment system using a Random Forest classifier, which may provide great qualitative classification accuracy. Furthermore, a novel functional score is generated from the probabilities belonging to the pathological group. Finally, A study involving ten healthy subjects and ten post-stroke patients validates the proposed method. The experimental results show that the classification accuracy was enhanced to 98.56% by fusing the characteristics derived from different domains, which was higher than that based on spatial domain (94.90%) and temporal domain (91.08%), respectively. Furthermore, the assessment score generated by multi-domain fusion framework exhibited a significant correlation with the clinical score. These promising results show the potential of applying the proposed method to clinical assessments for post-stroke patients.