Phyllostachys nigra (Lodd. ex Lindl.) derived polysaccharide with enhanced glycolipid metabolism regulation and mice gut microbiome

Kui Zhao; Xueyi Wu; Guiqi Han; Lin Sun; Changwen Zheng; Hua Hou; Ben Bin Xu; Zeinhom M El-Bahy; Cheng Qian; Mohamed Kallel; Hassan Algadi; Zhanhu Guo; Zhengjun Shi

doi:10.1016/j.ijbiomac.2023.128588

Phyllostachys nigra (Lodd. ex Lindl.) derived polysaccharide with enhanced glycolipid metabolism regulation and mice gut microbiome

Int J Biol Macromol. 2024 Feb;257(Pt 1):128588. doi: 10.1016/j.ijbiomac.2023.128588. Epub 2023 Dec 2.

Authors

Kui Zhao¹, Xueyi Wu², Guiqi Han³, Lin Sun⁴, Changwen Zheng³, Hua Hou⁵, Ben Bin Xu⁶, Zeinhom M El-Bahy⁷, Cheng Qian¹, Mohamed Kallel⁸, Hassan Algadi⁹, Zhanhu Guo¹⁰, Zhengjun Shi¹¹

Affiliations

¹ College of Material Science and Chemical Engineering, Southwest Forestry University, Kunming 650224, China.
² Department of Endocrinology, The Second People's Hospital of Guiyang, Guiyyang 550081, China.
³ State Administration of Traditional Chinese Medicine Key Laboratory of Traditional Chinese Medicine Regimen and Health, Chengdu, Sichuan 610075, China.
⁴ Faculty of Materials and Chemical Engineering, Yibin University, Yibin 644000, China.
⁵ College of Materials Science and Engineering, Taiyuan University of Science and Technology, Taiyuan 030024, China.
⁶ Department of Mechanical and Civil Engineering, Faculty of Engineering and Environment, Northumbria University, Newcastle Upon Tyne NE1 8ST, UK.
⁷ Department of Chemistry, Faculty of Science, Al-Azhar University, Nasr City 11884, Cairo, Egypt.
⁸ Department of Physics, Faculty of Sciences and Arts, Northern Border University, Rafha 91911, Saudi Arabia.
⁹ Department of Electrical Engineering, Faculty of Engineering, Najran University, Najran 11001, Saudi Arabia.
¹⁰ Department of Mechanical and Civil Engineering, Faculty of Engineering and Environment, Northumbria University, Newcastle Upon Tyne NE1 8ST, UK. Electronic address: zhanhu.guo@northumbria.ac.uk.
¹¹ College of Material Science and Chemical Engineering, Southwest Forestry University, Kunming 650224, China. Electronic address: shizhengjun1979@swfu.edu.cn.

PMID: 38048922
DOI: 10.1016/j.ijbiomac.2023.128588

Abstract

This study focuses on the characterization and regulation of glycolipid metabolism of polysaccharides derived from biomass of Phyllostachys nigra (Lodd. ex Lindl.) root (PNr). The extracts from dilute hydrochloric acid, hot water, and 2 % sodium hydroxide solution were characterized through molecular weight, gel permeation chromatography, monosaccharides, Fourier transform infrared, and nuclear magnetic resonance spectroscopy analyses. Polysaccharide from alkali extraction and molecular sieve purification (named as: PNS2A) exhibited optimal inhibitory of 3T3-L1 cellular differentiation and lowered insulin resistance. The PNS2A is made of a hemicellulose-like main chain of →4)-β-D-Xylp-(1→ that was connected by branches of 4-O-Me-α-GlcAp-(1→, T-α-D-Galp-(1→, T-α-L-Araf-(1→, →2)-α-L-Araf-(1→, as well as β-D-Glcp-(1→4-β-D-Glcp-(1→ fragments. Oral delivery of PNS2A in diabetes mice brought down blood glucose and cholesterol levels and regulated glucose and lipid metabolism. PNS2A alleviated diabetes symptoms and body weight and protected liver and kidney function in model animals by altering the gut microbiome. Polysaccharides can be a new approach to develop bamboo resources.

Keywords: Glycolipid metabolism; Gut microbiome; Phyllostachys nigra; Polysaccharides; Type 2 diabetes.

MeSH terms

Animals
Diabetes Mellitus*
Gastrointestinal Microbiome*
Glucose / analysis
Mice
Monosaccharides / analysis
Poaceae
Polysaccharides / chemistry

Substances

Polysaccharides
Monosaccharides
Glucose