Targeted amplification-based whole genome sequencing of Monkeypox virus in clinical specimens

S Isabel; A Eshaghi; V R Duvvuri; J B Gubbay; K Cronin; Aimin Li; M Hasso; S T Clark; J P Hopkins; S N Patel; T W A Braukmann

doi:10.1128/spectrum.02979-23

Targeted amplification-based whole genome sequencing of Monkeypox virus in clinical specimens

Microbiol Spectr. 2024 Jan 11;12(1):e0297923. doi: 10.1128/spectrum.02979-23. Epub 2023 Dec 4.

Authors

S Isabel^#¹, A Eshaghi^#¹, V R Duvvuri^{1

2}, J B Gubbay^{1

2}, K Cronin¹, Aimin Li¹, M Hasso¹, S T Clark¹, J P Hopkins^{1

3}, S N Patel^{1

2}, T W A Braukmann¹

Affiliations

¹ Public Health Ontario Laboratory, Public Health Ontario , Toronto, Ontario, Canada.
² Department of Laboratory Medicine and Pathobiology, Temerty Faculty of Medicine, University of Toronto , Toronto, Ontario, Canada.
³ Department of Health Research Methods, Evidence, and Impact, McMaster University , Hamilton, Ontario, Canada.

^# Contributed equally.

Abstract

We present a protocol to efficiently sequence genomes of the MPXV-causing mpox. This enables researchers and public health agencies to acquire high-quality genomic data using a rapid and cost-effective approach. Genomic data can be used to conduct surveillance and investigate mpox outbreaks. We present 91 mpox genomes that show the diversity of the 2022 mpox outbreak in Ontario, Canada.

Keywords: Monkeypox virus; WGS; mpox; targeted amplification.

MeSH terms

Disease Outbreaks
Genomics
Humans
Monkeypox virus* / genetics
Mpox (monkeypox)*
Ontario / epidemiology
Whole Genome Sequencing