Thermal and biological properties of novel sodium carboxymethylcellulose-PPFMA nanocomposites containing biosynthesized Ag-ZnO hybrid filler

Gofur Khamidov; Ömer Hazman; Ibrahim Erol

doi:10.1016/j.ijbiomac.2023.128447

Thermal and biological properties of novel sodium carboxymethylcellulose-PPFMA nanocomposites containing biosynthesized Ag-ZnO hybrid filler

Int J Biol Macromol. 2024 Feb;257(Pt 1):128447. doi: 10.1016/j.ijbiomac.2023.128447. Epub 2023 Nov 30.

Authors

Gofur Khamidov¹, Ömer Hazman², Ibrahim Erol³

Affiliations

¹ Samarkand State University, Institute of Biochemistry, Department of Organic Synthesis and Bioorganic Chemistry, University blvd-15, Samarkand, Uzbekistan.
² Samarkand State University, Institute of Biochemistry, Department of Organic Synthesis and Bioorganic Chemistry, University blvd-15, Samarkand, Uzbekistan; Afyon Kocatepe University, Faculty of Science and Arts, Department of Chemistry, 03200 Afyonkarahisar, Türkiye.
³ Samarkand State University, Institute of Biochemistry, Department of Organic Synthesis and Bioorganic Chemistry, University blvd-15, Samarkand, Uzbekistan; Samarkand State University, Institute of Biochemistry, Department of Polymer Chemistry and Chemical Technology, University Blvd-15, Samarkand, Uzbekistan. Electronic address: ierol@aku.edu.tr.

PMID: 38040162
DOI: 10.1016/j.ijbiomac.2023.128447

Abstract

The aim of this study was to produce new nanocomposites with antimicrobial, antioxidant and anticancer properties that can be used in biomedical research based on carboxymethyl cellulose (NaCMC) biopolymer. First, poly(2-oxo-2-(pentafluorophenoxy)ethyl-2-methylprop-2-enoate) (PPFMA) was synthesized and characterized by FTIR and NMR techniques. It was then blended with NaCMC by in situ/hydrothermal method to produce a semi-synthetic functional material. Changes in the FTIR data of the blend and the single Tg value from DSC confirmed the compatibility of the blend. To enhance the thermal and biological properties of the NaCMC-PPFMA blend, biosynthesized Ag-ZnONPs were hydrothermally incorporated into the blend at different weight ratios. The prepared materials were characterized by SEM, EDX, TEM, XRD and FTIR. The thermal stability of the materials was determined by thermogravimetric analysis (TGA), and glass transition temperatures (Tg) was determined by differential scanning calorimeter (DSC). The oxidant, antioxidant, antimicrobial, and cytotoxic properties of PPFMA, Ag-ZnONPs, PPFMA-NaCMC blend, and nanocomposites were investigated in detail. The total oxidant state (TOS) value of the NaCMC-PPFMA blend, which was 0.72 μmol equivalent H₂O₂/L, increased to 7.2-10.4 μmol equivalent H₂O₂/L with the addition of Ag-ZnONPs. Ag-ZnONPs decreased total antioxidant state (TAS) levels of the nanocomposites while increasing their oxidant activity. Therefore, an increase in the antimicrobial activity of the nanocomposites was observed. Adding Ag-ZnONPs to the NaCMC-PPFMA blend increased the thermal stability by 22 °C and the Tg value by 9 °C. Finally, the potential of Ag-ZnONPs containing nanocomposites in wound healing therapies was examined. The findings suggest that nanocomposites prepared by incorporating Ag-ZnONPs into the semi-synthetic NaCMC-PPFMA blend can be a source of bio-safe raw materials and can be used as potential wound healers.

Keywords: Antimicrobial activity; Caroboxymethyl cellulose; Methacrylate; Thermal properties.

MeSH terms

Anti-Bacterial Agents / chemistry
Anti-Bacterial Agents / pharmacology
Anti-Infective Agents* / pharmacology
Antioxidants / pharmacology
Carboxymethylcellulose Sodium / chemistry
Hydrogen Peroxide
Nanocomposites* / chemistry
Oxidants
Zinc Oxide* / chemistry

Substances

Carboxymethylcellulose Sodium
Zinc Oxide
Antioxidants
Hydrogen Peroxide
Anti-Infective Agents
Oxidants
Anti-Bacterial Agents