Type 2 diabetes linked FTO gene variant rs8050136 is significantly associated with gravidity in gestational diabetes in a sample of Bangladeshi women: Meta-analysis and case-control study

U S Mahzabin Amin; Tahia Anan Rahman; Mashfiqul Hasan; Tania Tofail; Muhammad Abul Hasanat; Zeba I Seraj; Md Salimullah

doi:10.1371/journal.pone.0288318

Type 2 diabetes linked FTO gene variant rs8050136 is significantly associated with gravidity in gestational diabetes in a sample of Bangladeshi women: Meta-analysis and case-control study

PLoS One. 2023 Nov 30;18(11):e0288318. doi: 10.1371/journal.pone.0288318. eCollection 2023.

Authors

U S Mahzabin Amin¹, Tahia Anan Rahman¹, Mashfiqul Hasan², Tania Tofail², Muhammad Abul Hasanat², Zeba I Seraj³, Md Salimullah¹

Affiliations

¹ Molecular Biotechnology Division, National Institute of Biotechnology (NIB), Savar, Dhaka, Bangladesh.
² Department of Endocrinology, Bangabandhu Sheikh Mujib Medical University (BSMMU), Dhaka, Bangladesh.
³ Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka, Bangladesh.

Abstract

Objective: Gestational diabetes mellitus (GDM) is a growing public health concern that has not been extensively studied. Numerous studies have indicated that a variant (rs8050136) of the fat mass-associated gene, FTO, is associated with both GDM and Type 2 diabetes mellitus(T2DM). We conducted a meta-analysis on the association between the FTO single nucleotide polymorphism (SNP) rs8050136 and T2DM, followed by a case-control study on the association of the said SNP and GDM in a sample of Bangladeshi women.

Method: A total of 25 studies were selected after exploring various databases and search engines, which were assessed using the Newcastle-Ottawa Scale (NOS). The MetaGenyo web tool was used to conduct this meta-analysis. A case-control study was performed on 218 GDM patients and 284 controls to observe any association between FTO rs8050136 and GDM. Genotyping was performed using the tetra-primer amplification refractory mutation system-polymerase chain reaction (T-ARMS) method, and statistical analyses were performed using various statistical softwares.

Results: In the meta-analysis 26231 cases and 43839 controls were examined. Pooled association analyses revealed a statistically significant relationship between the FTO rs8050136 polymorphism and an elevated risk of T2DM under all genetic models (P<0.05). In the case-control study, synergistic analyses of the SNP and gravida with GDM revealed a significant (P<0.01) association with an increase in odds by 1.6 to 2.4 folds in multigravida and decrease in odds by 2 folds in primigravida. A positive family history of diabetes and the minor allele of this SNP collectively increased the risk of developing GDM by many-fold (1.8 to 2.7 folds). However, after accounting for family history of diabetes and gravidity, analyses showed no significant association with GDM.

Conclusion: Our meta-analysis revealed a significant association between SNP rs8050136 of FTO with T2DM, and this variant was substantially associated with an increased risk of GDM in a sample of Bangladeshi multigravida women.

Copyright: © 2023 Amin et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Publication types

Meta-Analysis

MeSH terms

Alpha-Ketoglutarate-Dependent Dioxygenase FTO / genetics
Case-Control Studies
Diabetes Mellitus, Type 2* / genetics
Diabetes, Gestational* / genetics
Female
Genetic Predisposition to Disease
Gravidity
Humans
Polymorphism, Single Nucleotide
Pregnancy

Substances

Alpha-Ketoglutarate-Dependent Dioxygenase FTO
FTO protein, human

Grants and funding

The work has been supported by the fund of Special allocation of Ministry of Science and Technology(https://most.gov.bd/), Government of the people’s Republic of Bangladesh in the fiscal year of 2014-15. The project ID was MEDI; S- 19. SM received this fund. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.