Potential antioxidative and anti-hyperuricemic components in Rodgersia podophylla A. Gray revealed by bio-affinity ultrafiltration with SOD and XOD

Can Liang; Yongbing Xu; Minxia Fan; Felix Wambua Muema; Guilin Chen; Mingquan Guo; Guangwan Hu

doi:10.3389/fphar.2023.1298049

Potential antioxidative and anti-hyperuricemic components in Rodgersia podophylla A. Gray revealed by bio-affinity ultrafiltration with SOD and XOD

Front Pharmacol. 2023 Nov 8:14:1298049. doi: 10.3389/fphar.2023.1298049. eCollection 2023.

Authors

Can Liang^{1

2}, Yongbing Xu^{1

2}, Minxia Fan^{1

2

3}, Felix Wambua Muema^{1

2

3}, Guilin Chen^{1

2

3

4}, Mingquan Guo^{1

2}, Guangwan Hu^{1

2

3

4}

Affiliations

¹ Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, China.
² University of Chinese Academy of Sciences, Beijing, China.
³ Sino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan, China.
⁴ Hubei Jiangxia Laboratory, Wuhan, China.

Abstract

Rodgersia podophylla A. Gray (R. podophylla) is a traditional Chinese medicine with various pharmacological effects. However, its antioxidant and anti-hyperuricemia components and mechanisms of action have not been explored yet. In this study, we first assessed the antioxidant potential of R. podophylla with 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and ferric ion reducing antioxidant power (FRAP) assays. The results suggested that the ethyl acetate (EA) fraction of R. podophylla not only exhibited the strongest DPPH, ABTS radical scavenging and ferric-reducing activities, but also possessed the highest total phenolic and total flavonoid contents among the five fractions. After that, the potential superoxide dismutase (SOD) and xanthine oxidase (XOD) ligands from the EA fraction were quickly screened and identified through the bio-affinity ultrafiltration liquid chromatography-mass spectrometry (UF-LC-MS). Accordingly, norbergenin, catechin, procyanidin B2, 4-O-galloylbergenin, 11-O-galloylbergenin, and gallic acid were considered to be potential SOD ligands, while gallic acid, 11-O-galloylbergenin, catechin, bergenin, and procyanidin B2 were recognized as potential XOD ligands, respectively. Moreover, these six ligands effectively interacted with SOD in molecular docking simulation, with binding energies (BEs) ranging from -6.85 to -4.67 kcal/mol, and the inhibition constants (Ki) from 9.51 to 379.44 μM, which were better than the positive controls. Particularly, catechin exhibited a robust binding affinity towards XOD, with a BE value of -8.54 kcal/mol and Ki value of 0.55 μM, which surpassed the positive controls. In conclusion, our study revealed that R. podophylla possessed remarkable antioxidant and anti-hyperuricemia activities and that the UF-LC-MS method is suitable for screening potential ligands for SOD and XOD from medicinal plants.

Keywords: Rodgersia podophylla; UF-LC-MS; anti-hyperuricemic; antioxidative; superoxide dismutase; xanthine oxidase.

Grants and funding

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This research was funded by the Youth Innovation Promotion Association of Chinese Academy of Sciences (No. 2020337).