Effects of hydroxyapatite-coated porous titanium scaffolds functionalized by exosomes on the regeneration and repair of irregular bone

Hanyu Shao; Qiyue Zhang; Mingman Sun; Ming Wu; Xu Sun; Qiang Wang; Shuang Tong

doi:10.3389/fbioe.2023.1283811

Effects of hydroxyapatite-coated porous titanium scaffolds functionalized by exosomes on the regeneration and repair of irregular bone

Front Bioeng Biotechnol. 2023 Oct 31:11:1283811. doi: 10.3389/fbioe.2023.1283811. eCollection 2023.

Authors

Hanyu Shao^#¹, Qiyue Zhang^#², Mingman Sun¹, Ming Wu¹, Xu Sun¹, Qiang Wang², Shuang Tong¹

Affiliations

¹ Department of Plastic Surgery, First Hospital of China Medical University, Shenyang, China.
² Liaoning Provincial Key Laboratory of Oral Diseases, School and Hospital of Stomatology, China Medical University, Shenyang, China.

^# Contributed equally.

Abstract

As a traditional bone implant material, titanium (Ti) and its alloys have the disadvantages of lack of biological activity and susceptibility to stress shielding effect. Adipose stem cells (ADSCs) and exosomes were combined with the scaffold material in the current work to effectively create a hydroxyapatite (HA) coated porous titanium alloy scaffold that can load ADSCs and release exosomes over time. The composite made up for the drawbacks of traditional titanium alloy materials with higher mechanical characteristics and a quicker rate of osseointegration. Exosomes (Exos) are capable of promoting the development of ADSCs in porous titanium alloy scaffolds with HA coating, based on experimental findings from in vitro and in vivo research. Additionally, compared to pure Ti implants, the HA scaffolds loaded with adipose stem cell exosomes demonstrated improved bone regeneration capability and bone integration ability. It offers a theoretical foundation for the combined use of stem cell treatment and bone tissue engineering, as well as a design concept for the creation and use of novel clinical bone defect repair materials.

Keywords: adipose-derived stem cells; bone tissue engineering; exosomes; hydroxyapatite; porous titanium alloy scaffold.

Grants and funding

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This work was supported by the Natural Science Foundation of Liaoning Province (2019-BS-28).