External quality assessment for laboratories in pan-India ILI/SARI surveillance for simultaneous detection of influenza virus and SARS-CoV-2

Varsha Potdar; Neetu Vijay; Veena Vipat; Sheetal Jadhav; Nivedita Gupta; Neeraj Aggarwal

doi:10.3389/fpubh.2023.1274508

External quality assessment for laboratories in pan-India ILI/SARI surveillance for simultaneous detection of influenza virus and SARS-CoV-2

Front Public Health. 2023 Nov 13:11:1274508. doi: 10.3389/fpubh.2023.1274508. eCollection 2023.

Authors

Varsha Potdar¹, Neetu Vijay², Veena Vipat¹, Sheetal Jadhav¹, Nivedita Gupta², Neeraj Aggarwal²

Affiliations

¹ Indian Council of Medical Research-National Institute of Virology, Pune, India.
² Division of Epidemiology and Communicable Diseases, Indian Council of Medical Research, New Delhi, India.

Abstract

Introduction: The Indian Council of Medical Research has set up a nationwide network of 28 laboratories for simultaneous surveillance of influenza virus and SARS-CoV-2 in ILI/SARI patients, using an in-house developed and validated multiplex real-time RTPCR assay. The aim of this study was to ensure the quality of testing by these laboratories by implementing an external quality assessment program (EQAP).

Methods: For this EQAP, a proficiency test (PT) panel comprising tissue-culture or egg-grown influenza virus and SARS-CoV-2 was developed. The PT panel was distributed to all the participant laboratories, which tested the panel and submitted the qualitative results online to the EQAP provider. The performance of the laboratories was evaluated on qualitative criteria but cycle threshold (Ct) values were also gathered for each sample.

Results: On a qualitative basis, all the laboratories achieved the criteria of 90% concordance with the results of the PT panel provider. Ct values of different samples across the laboratories were within ≤ ±3 cycles of the corresponding mean values of the respective sample. The results of this EQAP affirmed the quality and reliability of testing being done for simultaneous surveillance of influenza virus and SARS-CoV-2 in India.

Keywords: EQAP; ILI; SARI; SARS-CoV-2; influenza; surveillance.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

COVID-19* / diagnosis
Humans
India
Influenza, Human* / diagnosis
Influenza, Human* / epidemiology
Laboratories
Orthomyxoviridae*
Reproducibility of Results
SARS-CoV-2

Grants and funding

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This ILI/SARI surveillance program is funded by the Department of Health Research, Ministry of Health and Family Welfare, New Delhi (Grant No. R.15013/01/2021-HR/VRDL).