Bacterial Nanocellulose/Copper as a Robust Laccase-Mimicking Bionanozyme for Catalytic Oxidation of Phenolic Pollutants

Afomiya Animaw Achamyeleh; Biniyam Abera Ankala; Yitayal Admassu Workie; Menbere Leul Mekonnen; Ebrahim M Abda

doi:10.1021/acsomega.3c06847

Bacterial Nanocellulose/Copper as a Robust Laccase-Mimicking Bionanozyme for Catalytic Oxidation of Phenolic Pollutants

ACS Omega. 2023 Nov 6;8(45):43178-43187. doi: 10.1021/acsomega.3c06847. eCollection 2023 Nov 14.

Authors

Afomiya Animaw Achamyeleh¹, Biniyam Abera Ankala², Yitayal Admassu Workie^{2

3}, Menbere Leul Mekonnen^{2

3}, Ebrahim M Abda^{1

4}

Affiliations

¹ Biotechnology Department, Addis Ababa Science and Technology University, Addis Ababa, P.O. Box 1647, Ethiopia.
² Industrial Chemistry Department, Addis Ababa Science and Technology University, Addis Ababa, P.O. Box 1647, Ethiopia.
³ Nanotechnology Center of Excellence, Addis Ababa Science and Technology University, Addis Ababa, P.O. Box 1647, Ethiopia.
⁴ Biotechnology and Bio-processing Center of Excellence, Addis Ababa Science and Technology University, Addis Ababa, P.O. Box 1647, Ethiopia.

Abstract

Industrial effluents containing phenolic compounds are a major public health concern and thus require effective and robust remediation technologies. Although laccase-like nanozymes are generally recognized as being catalytically efficient in oxidizing phenols, their support materials often lack resilience in harsh environments. Herein, bacterial nanocellulose (BNC) was introduced as a sustainable, strong, biocompatible, and environmentally friendly biopolymer for the synthesis of a laccase-like nanozyme (BNC/Cu). A native bacterial strain that produces nanocellulose was isolated from black tea broth fermented for 1 month. The isolate that produced BNC was identified as Bacillus sp. strain T15, and it can metabolize hexoses, sucrose, and less expensive substrates, such as molasses. Further, BNC/Cu nanozyme was synthesized using the in situ reduction of copper on the BNC. Characterization of the nanozyme by scanning electron microscopy (SEM) and X-ray diffraction (XRD) confirmed the presence of the copper nanoparticles dispersed in the layered sheets of BNC. The laccase-mimetic activity was assessed using the chromogenic redox reaction between 2,4-dichlorophenol (2,4-DP) and 4-aminoantipyrine (4-AP) with characteristic absorption at 510 nm. Remarkably, BNC/Cu has 50.69% higher catalytic activity than the pristine Cu NPs, indicating that BNC served as an effective biomatrix to disperse Cu NPs. Also, the bionanozyme showed the highest specificity toward 2,4-DP with a K_m of 0.187 mM, which was lower than that of natural laccase. The bionanozyme retained catalytic activity across a wider temperature range with optimum activity at 85 °C, maintaining 38% laccase activity after 11 days and 46.77% activity after the fourth cycle. The BNC/Cu bionanozyme could efficiently oxidize more than 70% of 1,4-dichlorophenol and phenol in 5 h. Thereby, the BNC/Cu bionanozyme is described here as having an efficient ability to mimic laccase in the oxidation of phenolic compounds that are commonly released into the environment by industry.