Reducing Dietary Protein Content by Increasing Carbohydrates Is More Beneficial to the Growth, Antioxidative Capacity, Ion Transport, and Ammonia Excretion of Nile Tilapia (Oreochromis niloticus) under Long-Term Alkalinity Stress

Wei Liu; Chang Xu; Zhao Li; Liqiao Chen; Xiaodan Wang; Erchao Li

doi:10.1155/2023/9775823

Reducing Dietary Protein Content by Increasing Carbohydrates Is More Beneficial to the Growth, Antioxidative Capacity, Ion Transport, and Ammonia Excretion of Nile Tilapia (Oreochromis niloticus) under Long-Term Alkalinity Stress

Aquac Nutr. 2023 Nov 16:2023:9775823. doi: 10.1155/2023/9775823. eCollection 2023.

Authors

Wei Liu¹, Chang Xu¹, Zhao Li¹, Liqiao Chen², Xiaodan Wang², Erchao Li^{1

2}

Affiliations

¹ Key Laboratory of Tropical Hydrobiology and Biotechnology of Hainan Province, Hainan Aquaculture Breeding Engineering Research Center, School of Marine Biology and Aquaculture, Hainan University, Haikou 570228, China.
² School of Life Sciences, East China Normal University, Shanghai 200241, China.

Abstract

Alkalinity stress is the main stress experienced by aquatic animals in saline-alkali water, which hinders the aquaculture development and the utilization of water resources. The two-factor (2 × 3) test was adopted to study the influence of dietary protein to carbohydrate ratios on the energy metabolism of Nile tilapia (Oreochromis niloticus) under different alkalinity stress levels. Three diets with different protein-carbohydrate ratios (P27/C35, P35/C25, and P42/C15) were fed to fish cultured in freshwater (FW, 1.3 mmol/L carbonate alkalinity) or alkaline water (AW, 35.7 mmol/L carbonate alkalinity) for 50 days. Ambient alkalinity decreased tilapia growth performance. Although ambient alkalinity caused oxidative stress and enhanced ion transport and ammonia metabolism in tilapia, tilapia fed the P27/C35 diet showed better adaptability than fish fed the other two diets in alkaline water. Further metabolomic analysis showed that tilapia upregulated all the pathways enriched in this study to cope with alkalinity stress. Under alkalinity stress, tilapia fed the P27/C35 diet exhibited enhanced pyruvate metabolism and purine metabolism compared with tilapia fed the P42/C15 diet. This study indicated that ambient alkalinity could significantly decrease growth performance and cause oxidative stress and osmotic regulation. However, reducing dietary protein content by increasing carbohydrates could weaken stress and improve growth performance, ion transport, and ammonia metabolism in tilapia under long-term hyperalkaline exposure.