Bacterial DNA and osteoarthritis in dogs with patellar luxation and cranial cruciate ligament rupture

Sirun Tuek-Um; Sarawut Yangtara; Win Surachetpong; Sarawan Kaewmongkol; Gunn Kaewmongkol; Naris Thengchaisri

doi:10.14202/vetworld.2023.2049-2054

Bacterial DNA and osteoarthritis in dogs with patellar luxation and cranial cruciate ligament rupture

Vet World. 2023 Oct;16(10):2049-2054. doi: 10.14202/vetworld.2023.2049-2054. Epub 2023 Oct 7.

Authors

Sirun Tuek-Um¹, Sarawut Yangtara², Win Surachetpong³, Sarawan Kaewmongkol⁴, Gunn Kaewmongkol², Naris Thengchaisri²

Affiliations

¹ Surgery Unit, Kasetsart University Veterinary Teaching Hospital, Bangkhen campus, Bangkok 10900, Thailand.
² Department of Companion Animal Clinical Sciences, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.
³ Department of Veterinary Microbiology and Immunology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.
⁴ Department of Veterinary Nursing, Faculty of Veterinary Technology, Kasetsart University, Bangkok, Thailand.

Abstract

Background and aim: The association between bacterial DNA in stifle joints, including those with cranial cruciate ligament rupture (CCLR) and medial patellar luxation (MPL), and osteoarthritis in dogs remains elusive. This study investigated the potential association between the detection of bacterial DNA and osteoarthritis in dogs using a broad-range polymerase chain reaction technique targeting the 16S ribosomal RNA gene.

Materials and methods: Synovial fluid (35 samples) and knee tissue samples (32 samples) were obtained from 35 dogs diagnosed with CCLR (n = 20; 11 males and nine females) or MPL (n = 15; five males and 10 females) who underwent a surgical operation between October 2014 and April 2015.

Results: Dogs with CCLR had a higher average osteoarthritis score than those with MPL (2.0 ± 0.9 vs. 0.5 ± 0.9; p = 0.005). Bacterial DNA was detected in the stifle joints of 60.71% of dogs with MPL. Pelomonas spp. (25.00%), Halomonas spp. (17.86%), and 5 other species (17.86%) were the most frequently identified bacteria. Bacterial DNA was detected in 41.03% of dogs with CCLR. Pelomonas spp. (15.38%), Sphingomonas spp. (10.26%), Halomonas spp. (5.13%), and 4 other species (10.26%) were the most frequently identified bacteria. No significant difference was observed in the prevalence of bacterial DNA obtained from tissue samples (46.88%) or joint fluid samples (51.43%). The presence of bacterial DNA was not associated with the type of knee injury (MPL or CCLR; p = 1.000). There was a higher prevalence of bacterial DNA in samples from dogs with moderate-to-severe osteoarthritis (94.44%) than in those with minimal osteoarthritis (41.18%), and a significant association between the presence of bacterial DNA and moderate-to-severe osteoarthritis was identified (p < 0.01).

Conclusion: Dogs with moderate-to-severe osteoarthritis were more likely to have bacterial DNA in their stifle joints than those with no or minimal osteoarthritis. These findings provide valuable insight into the potential role of bacterial DNA in joint tissue or joint fluid and the development of osteoarthritis in dogs.

Keywords: bacterial DNA; cranial cruciate ligament; osteoarthritis; stifle.