Synthesis of Zinc Oxide Nanoparticles From Aqueous Extract of Avicennia marina Mangrove Leaves and Their Antibacterial Activities Against Oral Pathogens

Abstract

Introduction The field of nanotechnology is currently being extensively researched. Nanoparticles (NPs) are used in many fields, such as engineering and medicine, owing to their nanoscale dimensions. Zinc (Zn) appears to be the most desirable metal NP, as it is being applied in various drug delivery systems and other fields. The green synthesis of the NPs used in this study makes it affordable and nonpolluting. Avicennia marina leaves possess antimicrobial properties and a high secondary metabolite content. This study aimed to synthesize ZnO NPs from the aqueous extracts of A. marina mangrove leaves and assess their antibacterial activities against oral pathogens. Methodology The leaves of A. marina were dried to obtain a preprocessed powder, and from that, an aqueous extract was prepared. ZnO NPs were then synthesized by adding the aqueous extract to 100 mL of ZnS solution and mixing it in an orbital shaker. They were observed both visually and by ultraviolet (UV) spectrophotometry to confirm their synthesis. The antibacterial properties of these ZnO NPs were assayed using the disc diffusion method on three different oral bacterial strains (Streptococcus mutans, Staphylococcus aureus, and Klebsiella sp.). Results For the synthesis process, it was seen that zinc oxide (ZnO) NPs exhibited a deepening in coloration. Additionally, the UV spectrum analysis revealed a notable absorbance value of 1.2 at a wavelength of 320 nm. The antibacterial efficacy against S. mutans, S. aureus, and Klebsiella sp. was assessed by measuring the zone of inhibition in diameter. At a dosage of 100 µg/mL of ZnO NPs, the inhibition zones were found to be 7.5 ± 0.2, 9.5 ± 0.5, and 9.5 ± 1.2 mm for S. mutans, S. aureus, and Klebsiella sp., respectively. Similarly, at a concentration of 75 µg/mL, the inhibition zones were measured to be 7 ± 0.25, 9 ± 1, and 7.5 ± 0.5 mm for the respective bacterial strains. Conclusions This study synthesizes ZnO NPs using A. marina leaf aqueous extract in a sustainable and eco-friendly manner. The ZnO NPs' antibacterial activities against oral infections indicate their use in dental products. These NPs have promising potential for nanomedicine and oral health studies due to their antibacterial properties and ecologically sustainable manufacturing.

Keywords: avicennia marina; biosynthesis; innovative technique; novel agents; oral pathogens; uv spectroscopy; zn nanoparticles.